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Volume 64, Issue Pt_9

sp. nov., isolated from soil of a bamboo grove Free

Abstract

A Gram-stain-positive, aerobic, motile by gliding, rod-shaped bacterial strain, THG-GM18, was isolated from soil of a bamboo grove. Strain THG-GM18 was able to grow in the presence of up to 6.0 % (w/v) NaCl, at 4–37 °C and at pH 7.0–10.0 in R2A medium. Based on 16S rRNA gene sequence similarity, strain THG-GM18 was closely related to species of the genus . The most closely related strains to strain THG-GM18 are CCM 1646 (98.5 % similarity), G2-1 (98.4 %), DSM 420 (98.2 %), DSM 20116 (98.1 %) and A6 (98.0 %). Strain THG-GM18 possessed chemotaxonomic properties consistent with those of members of the genus , such as peptidoglycan type A3α (-Lys–-Ala–-Thr–-Ala), MK-9 as major menaquinone and anteiso- and iso-branched compounds (anteiso-C, iso-C and anteiso-C) as major cellular fatty acids. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, unidentified phospholipids, unidentified aminolipids, an unidentified glycolipid and unidentified lipids. The G+C content of the genomic DNA was 61.0 mol%. The DNA–DNA relatedness values between strain THG-GM18 and its closest phylogenetic neighbours were below 26.0 %. The results of physiological and biochemical tests allowed the differentiation of strain THG-GM18 from species of the genus with validly published names. sp. nov. is the proposed name, and the type strain is THG-GM18 ( = KACC 17531 = JCM 19335).

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Funding
This study was supported by the:
  • Kyung Hee University (Award 20120115)
© 2014 IUMS
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2014-09-01
2024-05-01
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References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  2. Busse H. J., Denner E. B., Lubitz W. ( 1996 ). Classification and identification of bacteria: current approaches to an old problem. Overview of methods used in bacterial systematics. . J Biotechnol 47, 3–38. [View Article] [PubMed]
    [Google Scholar]
  3. Cappuccino J. G., Sherman N. ( 2002 ). Microbiology: a Laboratory Manual, , 6th edn.. San Francisco:: Pearson Education;.
     [Google Scholar]
  4. Chen Y. G., Tang S. K., Zhang Y. Q., Li Z. Y., Yi L. B., Wang Y. X., Li W. J., Cui X. L. ( 2009 ). Arthrobacter halodurans sp. nov., a new halotolerant bacterium isolated from sea water. . Antonie van Leeuwenhoek 96, 63–70. [View Article] [PubMed]
    [Google Scholar]
  5. Conn H. J., Dimmick I. ( 1947 ). Soil bacteria similar in morphology to Mycobacterium and Corynebacterium . . J Bacteriol 54, 291–303.[PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limit on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  8. Ganzert L., Bajerski F., Mangelsdorf K., Lipski A., Wagner D. ( 2011 ). Arthrobacter livingstonensis sp. nov. and Arthrobacter cryotolerans sp. nov., salt-tolerant and psychrotolerant species from Antarctic soil. . Int J Syst Evol Microbiol 61, 979–984. [View Article] [PubMed]
    [Google Scholar]
  9. Hall T. A. ( 1999 ). BioEdit: a user–friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucl Acids Symp Ser 41, 95–98.
     [Google Scholar]
  10. Hiraishi A., Ueda Y., Ishihara J., Mori T. ( 1996 ). Comparative lipoquinone analysis of influent sewage and activated sludge by high–performance liquid chromatography and photodiode array detection. . J Gen Appl Microbiol 42, 457–469. [View Article]
    [Google Scholar]
  11. Jensen V. ( 1960 ). Arthrobacter ramosus spec. nov. A new Arthrobacter species isolated from forest soils. . Arsskr K Vet Landbohojsk 1960, 123–132.
     [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon–e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  13. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  14. Koch C., Schumann P., Stackebrandt E. ( 1995 ). Reclassification of Micrococcus agilis (Ali-Cohen 1889) to the genus Arthrobacter as Arthrobacter agilis comb. nov. and emendation of the genus Arthrobacter. . Int J Syst Bacteriol 45, 837–839. [View Article] [PubMed]
    [Google Scholar]
  15. Kodama Y., Yamamoto H., Amano N., Amachi T. ( 1992 ). Reclassification of two strains of Arthrobacter oxydans and proposal of Arthrobacter nicotinovorans sp. nov. . Int J Syst Bacteriol 42, 234–239.[PubMed] [CrossRef]
    [Google Scholar]
  16. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  17. Kotoucková L., Schumann P., Durnová E., Spröer C., Sedlácek I., Neca J., Zdráhal Z., Nemec M. ( 2004 ). Arthrobacter nitroguajacolicus sp. nov., a novel 4–nitroguaiacol–degrading actinobacterium. . Int J Syst Evol Microbiol 54, 773–777. [View Article] [PubMed]
    [Google Scholar]
  18. Kumar S., Nei M., Dudley J., Tamura K. ( 2008 ). MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9, 299–306. [View Article] [PubMed]
    [Google Scholar]
  19. Margesin R., Schumann P., Zhang D. C., Redzic M., Zhou Y. G., Liu H. C., Schinner F. ( 2012 ). Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite. . Int J Syst Evol Microbiol 62, 397–402. [View Article] [PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high–performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  21. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104–117. [View Article]
    [Google Scholar]
  22. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parleet J. H. ( 1984 ). An intergrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  23. Phillips H. C. ( 1953 ). Characterization of the soil globiforme bacteria. . Iowa State J Sci 27, 240–241.
     [Google Scholar]
  24. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  25. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
     [Google Scholar]
  26. Schenkel E., Berlaimont V., Dubois J., Helson-Cambier M., Hanocq M. ( 1995 ). Improved high-performance liquid chromatographic method for the determination of polyamines as their benzoylated derivatives: application to P388 cancer cells. . J Chromatogr B Biomed Appl 668, 189–197. [View Article] [PubMed]
    [Google Scholar]
  27. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407–477.[PubMed]
    [Google Scholar]
  28. Schumann P. ( 2011 ). Peptidoglycan structure. . Methods Microbiol 38, 101–129. [View Article]
    [Google Scholar]
  29. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic Note: A place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  30. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226–231.[PubMed]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  32. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;. [View Article]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  34. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697–703.[PubMed]
    [Google Scholar]
  35. Westerberg K., Elväng A. M., Stackebrandt E., Jansson J. K. ( 2000 ). Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol. . Int J Syst Evol Microbiol 50, 2083–2092. [View Article] [PubMed]
    [Google Scholar]
  36. Yassin A. F., Sproer C., Siering C., Hupfer H., Schumann P. ( 2011 ). Arthrobacter equi sp. nov., isolated from veterinary clinical material. . Int J Syst Evol Microbiol 61, 2089–2094. [View Article] [PubMed]
    [Google Scholar]
  37. Zhang J., Ma Y., Yu H. ( 2012 ). Arthrobacter cupressi sp. nov., an actinomycete isolated from the rhizosphere soil of Cupressus sempervirens. . Int J Syst Evol Microbiol 62, 2731–2736. [View Article] [PubMed]
    [Google Scholar]
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Arthrobacter bambusae sp. nov., isolated from soil of a bamboo grove
Int J Syst Evol Microbiol 64, 3069 (2014); https://doi.org/10.1099/ijs.0.064550-0
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