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Volume 64, Issue Pt_9

sp. nov., a bacteriocin-producing streptomycete that inhibits meticillin-resistant Free

Abstract

Two novel actinobacteria, strains MUSC 135 and MUSC 137, were isolated from mangrove soil at Tanjung Lumpur, Malaysia. The 16S rRNA gene sequence similarity and DNA–DNA relatedness between strains MUSC 135 and MUSC 137 were 100 % and 83±3.2 %, confirming that these two strains should be classified in the same species. Strain MUSC 135 exhibited a broad-spectrum bacteriocin against the pathogens meticillin-resistant (MRSA) strain ATCC BAA-44, ATCC 19430 and ATCC 7966. A polyphasic approach was used to study the taxonomy of MUSC 135, and it showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus . The diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). Polar lipids detected were a lipid, an aminolipid, a phospholipid, phosphatidylinositol, phosphatidylethanolamine and two glycolipids. The predominant cellular fatty acids (>10.0 %) were anteiso-C (20.8 %), iso-C (18.0 %), iso-C (12.2 %) and anteiso-C (11.6 %). The whole-cell sugars were ribose, glucose and mannose. These results suggested that MUSC 135 should be placed within the genus . Phylogenetic analysis based on the 16S rRNA gene sequence exhibited that the most closely related strains were NBRC 15397 (99.18 % similarity), NBRC 100915 (99.17 %) and NBRC 12757 (98.97 %). DNA–DNA relatedness between MUSC 135 and closely related type strains ranged from 26.3±2.1 to 49.6±2.5 %. BOX-PCR fingerprint comparisons showed that MUSC 135 exhibited a unique DNA profile. The DNA G+C content determined was 70.7±0.3 mol%. Based on our polyphasic study of MUSC 135, the strain merits assignment to a novel species, for which the name sp. nov. is proposed. The type strain is MUSC 135 ( = MCCC 1K00252 = DSM 42140).

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Funding
This study was supported by the:
  • University of Malaya for High Impact Research Grant (Award UM-MOHE HIR Nature Microbiome grant no. H-50001-A000027)
  • External Industry Grant from Biotek Abadi Sdn Bhd (Award GBA-808138)
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2014-09-01
2024-04-24
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Streptomyces pluripotens sp. nov., a bacteriocin-producing streptomycete that inhibits meticillin-resistant Staphylococcus aureus
Int J Syst Evol Microbiol 64, 3297 (2014); https://doi.org/10.1099/ijs.0.065045-0
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