Brenneria populi sp. nov., isolated from symptomatic bark of Populus×euramericana canker
Li, Yong and Fang, Wei and Xue, Han and Liang, Wen-xing and Wang, Lai-fa and Tian, Guo-zhong and Wang, Xi-zhuo and Lin, Cai-li and Li, Xia and Piao, Chun-gen,, 65, 432-437 (2015),
doi = https://doi.org/10.1099/ijs.0.066068-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Five Gran-stain-negative, facultatively anaerobic, motile, bacterial strains were isolated from symptomatic bark tissue of Populus×euramericana canker. Strains grew at 4–41 °C, pH 4–10 and 0–6 % (w/v) salinity. They were positive with respect to catalase activity and negative for oxidase activity, nitrate reduction and the Voges–Proskauer reaction. Analysis of 16S rRNA gene sequences indicated that these five poplar isolates belong to the genus Brenneria , having highest sequence similarity of 95.98 % with Brenneria goodwinii LMG 26270T. These five isolates formed a single cluster based on multilocus sequence analysis, indicating that they all belong to a single taxon within the genus Brenneria , which was confirmed by DNA–DNA hybridization. The DNA G+C content was 54.9–55.7 mol%, and the main fatty acids were C16 : 0, C18 : 1ω7c, C17 : 0 cyclo and C16 : 1ω7c/iso-C15 : 0 2-OH. Based on these results, we describe a novel species of the genus Brenneria with the proposed name Brenneria populi sp. nov. The type strain is D9-5T ( = CFCC 11963T = KCTC 42088T).,
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