1887

Abstract

During the study of bacteria associated with bats affected by white-nose syndrome hibernating in caves in the Czech Republic, we isolated two facultatively anaerobic, Gram-stain-negative bacteria, designated strains 12 and 52. Strains 12 and 52 were motile, rod-like bacteria (0.5–0.6 µm in diameter; 1–1.3 µm long), with optimal growth at 20–35 °C and pH 6–8. On the basis of the almost complete sequence of their 16S rRNA genes they should be classified within the genus ; the closest relatives to strains 12 and 52 were DSM 4597 (99.5 % similarity in 16S rRNA gene sequences) and DSM 4569 (99.5 % similarity in 16S rRNA gene sequences), respectively. DNA–DNA relatedness between strain 12 and DSM 4597 was only 37.1 % and between strain 52 and DSM 4569 was only 56.2 %. Both values are far below the 70 % threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus as representatives of sp. nov. (type strain 12 = CECT 8594 = DSM 28726) and sp. nov. (type strain 52 = CECT 8595 = DSM 28727).

Funding
This study was supported by the:
  • European Social Fund
  • Czech Republic (Award CZ.1.07/2.3.00/30.0003)
  • Grant Agency of the Czech Republic (Award P506/12/1064)
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2015-01-01
2024-03-28
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410. [View Article] [PubMed]
    [Google Scholar]
  2. Barnum D. A., Thackeray E. L., Fish N. A. ( 1958 ). An outbreak of mastitis caused by Serratia marcescens . . Can J Comp Med Vet Sci 22, 392395.[PubMed]
    [Google Scholar]
  3. Brisou B., Richard C., Lenriot A. ( 1972 ). Intérêt taxonomique de la recherche de la -xylosidase chez les Enterobacteriaceae . . Ann Inst Pasteur (Paris) 123, 341347.[PubMed]
    [Google Scholar]
  4. Carter G. R. ( 1973 ). Diagnostic Procedures in Veterinary Microbiology. Springfield, IL:: Charles C. Thomas;.
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  7. Deom J., Mortelmans J. ( 1953 ). Etude d’une souche pathogène de Serratia marcescens . . Rev Immunol (Paris) 17, 394398.
    [Google Scholar]
  8. Doetsch R. N. ( 1981 ). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 2133. Edited by Gerdhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Geiger A., Fardeau M. L., Falsen E., Ollivier B., Cuny G. ( 2010 ). Serratia glossinae sp. nov., isolated from the midgut of the tsetse fly Glossina palpalis gambiensis . . Int J Syst Evol Microbiol 60, 12611265. [View Article] [PubMed]
    [Google Scholar]
  10. Grimont F., Grimont P. A. D. ( 2006 ). The genus Serratia . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 6, pp. 219244. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer; . [View Article]
    [Google Scholar]
  11. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  13. Klite P. D. ( 1965 ). Intestinal bacterial flora and transit time of three neotropical bat species. . J Bacteriol 90, 375379.[PubMed]
    [Google Scholar]
  14. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195206. [View Article]
    [Google Scholar]
  15. Mühldorfer K., Speck S., Kurth A., Lesnik R., Freuling C., Müller T., Kramer-Schadt S., Wibbelt G. ( 2011 ). Diseases and causes of death in European bats: dynamics in disease susceptibility and infection rates. . PLoS ONE 6, e29773. [View Article] [PubMed]
    [Google Scholar]
  16. Rivas R., García-Fraile P., Mateos P. F., Martínez-Molina E., Velázquez E. ( 2007 ). Characterization of xylanolytic bacteria present in the bract phyllosphere of the date palm Phoenix dactylifera . . Lett Appl Microbiol 44, 181187. [View Article] [PubMed]
    [Google Scholar]
  17. Rogers J. S., Swofford D. L. ( 1998 ). A fast method for approximating maximum likelihoods of phylogenetic trees from nucleotide sequences. . Syst Biol 47, 7789. [View Article] [PubMed]
    [Google Scholar]
  18. Roussel A., Lucas A., Bouley G. ( 1969 ). Serratia marcescens (Bacillus prodigiosus), une bactérie de la pathologie comparée. . Revue de Pathologie Comparée et de Médecine Expérimentale 6, 2729.
    [Google Scholar]
  19. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  23. Wijewanta E. A., Fernando M. ( 1970 ). Infection in goats owing to Serratia marcescens . . Vet Rec 87, 282284. [View Article] [PubMed]
    [Google Scholar]
  24. Wilson C. D. ( 1963 ). The microbiology of bovine mastitis in Great Britain. . Bull Off Int Epizoot 60, 533551.
    [Google Scholar]
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