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Volume 64, Issue Pt_12

sp. nov., an anaerobic, psychrophilic bacterium isolated from subseafloor sediment, and emended description of the genus Free

Abstract

An anaerobic, psychrophilic bacterium, strain MO-SPC2, was isolated from a methanogenic microbial community in a continuous-flow bioreactor that was established from subseafloor sediments collected from off the Shimokita Peninsula of Japan in the north-western Pacific Ocean. Cells were pleomorphic: spherical, annular, curved rod, helical and coccoid cell morphologies were observed. Motility only occurred in helical cells. Strain MO-SPC2 grew at 0–17 °C (optimally at 9 °C), at pH 6.0–8.0 (optimally at pH 6.8–7.2) and in 20–40 g NaCl l (optimally at 20–30 NaCl l). The strain grew chemo-organotrophically with mono-, di- and polysaccharides. The major end products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. The abundant polar lipids of strain MO-SPC2 were phosphatidylglycolipids, phospholipids and glycolipids. The major cellular fatty acids were C, C and Cω9. Isoprenoid quinones were not detected. The G+C content of the DNA was 32.3 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain MO-SPC2 was affiliated with the genus within the phylum , and its closest relatives were Grapes (88.4 % sequence identity), Buddy (86.7 %) and SPN1 (85.4 %). Based on phenotypic characteristics and phylogenetic traits, strain MO-SPC2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MO-SPC2 ( = JCM 17281 = DSM 23952). An emended description of the genus is also proposed.

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Funding
This study was supported by the:
  • Japan Society for the Promotion of Science
  • Ministry of Education, Culture, Sports, Science and Technology, Japan
  • US National Science Foundation (Award 0919251)
© 2014 IUMS
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2014-12-01
2024-04-16
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Sphaerochaeta multiformis sp. nov., an anaerobic, psychrophilic bacterium isolated from subseafloor sediment, and emended description of the genus Sphaerochaeta
Int J Syst Evol Microbiol 64, 4147 (2014); https://doi.org/10.1099/ijs.0.068148-0
/content/journal/ijsem/10.1099/ijs.0.068148-0
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