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Volume 64, Issue Pt_11

sp. nov. and sp. nov., endemic yeasts associated with flowers in South Africa Free

Abstract

In a taxonomic study of yeasts recovered from nectar of flowers and associated insects in South Africa, 11 strains were found to represent two novel species. Morphological and physiological characteristics and sequence analyses of the large-subunit rRNA gene D1/D2 region, as well as the actin, RNA polymerase II and elongation factor 2 genes, showed that the two novel species belonged to the genus . sp. nov. (type strain EBD-CdVSA09-2 = CBS 13649 = NRRL Y-63721; MycoBank no. MB809688; allotype EBD-CdVSA10-2 = CBS13650 = NRRL Y-63720) was recovered from nectar of and the beetle sp. This species belongs to the large-spored clade and is closely related to , with which mating reactions and single-spored asci were observed. sp. nov. (type strain EBD-CdVSA08-1 = CBS 13651 = NRRL Y-63722; MycoBank no. MB809689; allotype EBD-CdVSA57-2 = CBS 13729 = NRRL Y-63723) was isolated from nectar of , and and a honeybee, and is a sister species to and . Analyses of the four sequences demonstrated the existence of three separate phylotypes. Intraspecies matings led to the production of mature asci of unprecedented morphology, with a long, flexuous tail. A single ascospore was produced in all compatible crosses, regardless of sequence phylotype. The two species appear to be endemic to South Africa. The ecology and habitat specificity of these novel species are discussed in terms of host plant and insect host species.

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Funding
This study was supported by the:
  • Natural Science and Engineering Research Council of Canada
  • Spanish Ministry of Economy and Competitiveness through the Severo Ochoa Programme for Centres of Excellence in R&D&I (Award SEV-2012-0262)
  • Junta de Andalucía (Award P09-RNM-4517)
  • Spanish National Research Council (CSIC) – European Social funds (JAE-DOC Programme)
© 2014 IUMS
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2014-11-01
2024-04-23
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Metschnikowia drakensbergensis sp. nov. and Metschnikowia caudata sp. nov., endemic yeasts associated with Protea flowers in South Africa
Int J Syst Evol Microbiol 64, 3724 (2014); https://doi.org/10.1099/ijs.0.068445-0
/content/journal/ijsem/10.1099/ijs.0.068445-0
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