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Abstract

A Gram-stain-negative, variably shaped, non-flagellated, yellow-pigmented, aerobic bacterium, designated strain SGM2-10, was isolated from a paddy soil sample from the Suwon region, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was related most closely to RHYL-37. Strain SGM2-10 showed highest 16S rRNA gene sequence similarity to RHYL-37 (95.1 %), HY-50R (95.0 %) and JCN-23 (94.4 %). No other species in the family shared more than 94.1 % 16S rRNA gene sequence similarity with strain SGM2-10. The major fatty acids of strain SGM2-10 were iso-C, iso-C G and iso-C 3-OH. The only menaquinone was MK-7. The polar lipids comprised phosphatidylethanolamine, seven unknown lipids and ten unknown aminolipids. The G+C content of the DNA of strain SGM2-10 was 46.7 mol%. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain SGM2-10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SGM2-10 ( = KACC 17341 = JCM 19942). The description of the genus has also been emended.

Funding
This study was supported by the:
  • National Academy of Agricultural Science, Rural Development Administration, Republic of Korea
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/content/journal/ijsem/10.1099/ijs.0.068692-0
2015-01-01
2024-03-28
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References

  1. Breznak J. A., Costilow R. N. ( 1994 ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  2. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  3. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  4. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  6. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  7. Pruesse E., Peplies J., Glöckner F. O. ( 2012 ). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28, 18231829. [View Article] [PubMed]
    [Google Scholar]
  8. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  9. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  10. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  11. Zhang K., Tang Y., Zhang L., Dai J., Wang Y., Luo X., Liu M., Luo G., Fang C. ( 2009 ). Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil. . Int J Syst Evol Microbiol 59, 30583062. [View Article] [PubMed]
    [Google Scholar]
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