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Volume 64, Issue Pt_12

sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium from deep-sea sediment and reclassification of as comb. nov. Free

Abstract

A taxonomic study was carried out on strain P73, which was isolated from deep-sea sediment of the Indian Ocean by enrichment of polycyclic aromatic hydrocarbons. The strain was able to degrade biphenyl, naphthalene, 2-methylnaphthalene, 2,6-dimethylnaphthalene, acenaphthene, anthracene, phenanthrene, dibenzothiophene, dibenzofuran, fluorene, 4-methyldibenzothiophene and fluoranthene, but not pyrene or chrysene. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain P73 formed a clade with the genera and within the family , with highest sequence similarity of 96.98 % to H 14, followed by ZXM137 (96.42 %). The bacterium was Gram-stain-negative, oxidase- and catalase-positive, rod-shaped and non-motile. Growth was observed at salinities from 0.5 to 12 % and at temperatures from 10 to 41 °C. The principal fatty acids (>10 %) of strain P73 were summed feature 8 (Cω7/ω6) and Cω8 cyclo. The sole respiratory quinone was Q-10. The major lipids were phosphatidylglycerol, one unknown aminolipid, one unknown phospholipid and one unknown lipid; a second unknown phospholipid and one unknown glycolipid were present as minor components. The G+C content of the chromosomal DNA was 66.0 mol%. The combined genotypic and phenotypic data show that strain P73 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P73 ( = MCCC 1A01112 = LMG 27600 = DSM 27257). Phylogenetic study and existing phenotypic information also show that should be transferred to the genus as comb. nov. (type strain ZXM137 = MCCC 1A06432 = CGMCC 1.8891 = LMG 24854).

  • Published Online:
Funding
This study was supported by the:
  • COMRA (Award DY125-15-R-01)
  • Natural Science Foundation of China (Award 41276005 and 41076105)
  • National Infrastructure of Microbial Resources of China (Award NIMR-2014-9 and NIMR-2013-9)
© 2014 IUMS
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2014-12-01
2024-04-16
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Celeribacter indicus sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium from deep-sea sediment and reclassification of Huaishuia halophila as Celeribacter halophilus comb. nov.
Int J Syst Evol Microbiol 64, 4160 (2014); https://doi.org/10.1099/ijs.0.069039-0
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