1887

Abstract

Three strains of facultatively aerobic, moderately thermophilic bacteria were isolated from terrestrial hot springs in Baikal Lake region and Kamchatka (Russia). Cells of the new isolates were cocci reproducing by binary fission. The temperature range for growth was between 20 and 56 °C and the pH range for growth from pH 4.5 to 8.5, with optimal growth at 47–50 °C and pH 7.0–7.5. The organisms were chemoheterotrophs preferring sugars and polysaccharides as growth substrates. 16S rRNA gene sequences of strains 2842, 2813 and 2918Kr were nearly identical (99.7–100 % similarity) and indicated that the strains belonged to the phylum . The phylogenetically closest cultivated relatives were 06SJR6-2 and FYK2301M01 with 16S rRNA gene sequence similarity values of 82.4 and 80.3 %, respectively. The novel strains differed from them by higher growth temperature, sensitivity to NaCl concentration above 3.0 % and by their cellular fatty acids profile. On the basis of phylogenetic and physiological data, strains 2842, 2813 and 2918Kr represent a novel genus and species for which we propose the name sp. nov. The type strain is 2842 ( = VKM B-2832 = JCM 19875). We also propose that gen. nov. is the type genus of a novel family, fam. nov. and a novel order, ord. nov.

Funding
This study was supported by the:
  • EC FP7
  • Russian Foundation of Basic Research (Award 13-04-01310)
  • President of Russia (Award MK-6339.2013.4)
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2015-02-01
2024-03-29
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References

  1. Anisimova M., Gil M., Dufayard J. F., Dessimoz C., Gascuel O. ( 2011 ). Survey of branch support methods demonstrates accuracy, power, and robustness of fast likelihood-based approximation schemes. . Syst Biol 60, 685699. [View Article] [PubMed]
    [Google Scholar]
  2. Bonch-Osmolovskaya E. A., Sokolova T. G., Kostrikina N. A., Zavarzin G. A. ( 1990 ). Desulfurella acetovorans gen. nov., sp. nov. – a new thermophilic sulfur-reducing eubacterium. . Arch Microbiol 153, 151155. [View Article]
    [Google Scholar]
  3. Christie W. W., Sébédio J. L., Juanéda P. ( 2001 ). A practical guide to the analysis of conjugated linoleic acid (CLA). . Inform 12, 147152.
    [Google Scholar]
  4. Cole J. K., Gieler B. A., Heisler D. L., Palisoc M. M., Williams A. J., Dohnalkova A. C., Ming H., Yu T. T., Dodsworth J. A. & other authors ( 2013 ). Kallotenue papyrolyticum gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage (Kallotenuales ord. nov., Kallotenuaceae fam. nov.) within the class Chloroflexia . . Int J Syst Evol Microbiol 63, 46754682. [View Article] [PubMed]
    [Google Scholar]
  5. DeSantis T. Z., Hugenholtz P., Larsen N., Rojas M., Brodie E. L., Keller K., Huber T., Dalevi D., Hu P., Andersen G. L. ( 2006 ). Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with arb . . Appl Environ Microbiol 72, 50695072. [View Article] [PubMed]
    [Google Scholar]
  6. Fukunaga Y., Kurahashi M., Sakiyama Y., Ohuchi M., Yokota A., Harayama S. ( 2009 ). Phycisphaera mikurensis gen. nov., sp. nov., isolated from a marine alga, and proposal of Phycisphaeraceae fam. nov., Phycisphaerales ord. nov. and Phycisphaerae classis nov. in the phylum Planctomycetes . . J Gen Appl Microbiol 55, 267275. [View Article] [PubMed]
    [Google Scholar]
  7. Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O. ( 2010 ). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. . Syst Biol 59, 307321. [View Article] [PubMed]
    [Google Scholar]
  8. Kawaichi S., Ito N., Kamikawa R., Sugawara T., Yoshida T., Sako Y. ( 2013 ). Ardenticatena maritima gen. nov., sp. nov., a ferric iron- and nitrate-reducing bacterium of the phylum ‘Chloroflexi’ isolated from an iron-rich coastal hydrothermal field, and description of Ardenticatenia classis nov.. Int J Syst Evol Microbiol 63, 29923002. [View Article] [PubMed]
    [Google Scholar]
  9. Kevbrin V. V., Zavarzin G. A. ( 1992 ). The effect of sulfur compounds on growth of halophilic homoacetic bacterium Acetohalobium arabaticum . . Microbiology (English translation of Mikrobiologiya) 61, 563567.
    [Google Scholar]
  10. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115177. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  11. Lebedinsky A. V., Chernyh N. A., Bonch-Osmolovskaya E. A. ( 2007 ). Phylogenetic systematics of microorganisms inhabiting thermal environments. . Biochemistry (Mosc) 72, 12991312. [View Article] [PubMed]
    [Google Scholar]
  12. Li W., Godzik A. ( 2006 ). Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. . Bioinformatics 22, 16581659. [View Article] [PubMed]
    [Google Scholar]
  13. Liu D., Liu Y., Men X., Guo Q., Guo R., Qiu Y. ( 2012 ). [Isolation and characterization of Thermopirellula anaerolimosa gen. nov., sp. nov., an obligate anaerobic hydrogen-producing bacterium of the phylum Planctomycetes]. . Wei Sheng Wu Xue Bao 52, 9941001 (in Chinese).[PubMed]
    [Google Scholar]
  14. Losey N. A., Stevenson B. S., Busse H.-J., Sinninghe Damsté J. S., Rijpstra W. I., Rudd S., Lawson P. A. ( 2013 ). Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring. . Int J Syst Evol Microbiol 63, 41494157. [View Article] [PubMed]
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  16. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  17. Podosokorskaya O. A., Kadnikov V. V., Gavrilov S. N., Mardanov A. V., Merkel A. Y., Karnachuk O. V., Ravin N. V., Bonch-Osmolovskaya E. A., Kublanov I. V. ( 2013 ). Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae . . Environ Microbiol 15, 17591771. [View Article] [PubMed]
    [Google Scholar]
  18. Quast C., Pruesse E., Yilmaz P., Gerken J., Schweer T., Yarza P., Peplies J., Glöckner F. O. ( 2013 ). The silva ribosomal RNA gene database project: improved data processing and web-based tools. . Nucleic Acids Res 41 (Database issue), D590D596. [View Article] [PubMed]
    [Google Scholar]
  19. Ronquist F., Teslenko M., van der Mark P., Ayres D. L., Darling A., Höhna S., Larget B., Liu L., Suchard M. A., Huelsenbeck J. P. ( 2012 ). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. . Syst Biol 61, 539542. [View Article] [PubMed]
    [Google Scholar]
  20. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark:: MIDI Inc;.
    [Google Scholar]
  21. Slobodkina G. B., Panteleeva A. N., Kostrikina N. A., Kopitsyn D. S., Bonch-Osmolovskaya E. A., Slobodkin A. I. ( 2013 ). Tepidibacillus fermentans gen. nov., sp. nov.: a moderately thermophilic anaerobic and microaerophilic bacterium from an underground gas storage. . Extremophiles 17, 833839. [View Article] [PubMed]
    [Google Scholar]
  22. Slobodkina G. B., Kovaleva O. L., Miroshnichenko M. L., Slobodkin A. I., Kolganova T. V., Novikov A. A., van Heerden E., Bonch-Osmolovskaya E. A. ( 2014 ). Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., novel thermophilic anaerobic representatives of the phylum Planctomycetes.. IJSEM (in press).
    [Google Scholar]
  23. Wolin E. A., Wolin M. J., Wolfe R. S. ( 1963 ). Formation of methane by bacterial extracts. . J Biol Chem 238, 28822886.[PubMed]
    [Google Scholar]
  24. Yoon J., Jang J. H., Kasai H. ( 2014 ). Algisphaera agarilytica gen. nov., sp. nov., a novel representative of the class Phycisphaerae within the phylum Planctomycetes isolated from a marine alga. . Antonie van Leeuwenhoek 105, 317324. [View Article] [PubMed]
    [Google Scholar]
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