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Volume 65, Issue Pt_1

Proposal of sp. nov., isolated from the rhizosphere Free

Abstract

A yellow, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain JM-1) was isolated from the rhizosphere of a field-grown plant in Auburn, AL, USA. 16S rRNA gene sequence analysis of strain JM-1 showed high sequence similarity to the type strains of (98.9 %), (97.4 %), (97.3 %) and (97.1 %); sequence similarities to all other type strains of species of the genus were below 97.0 %. DNA–DNA hybridizations of strain JM-1 and DSM 30196, SMCC F199 and SMCC B0478 showed low similarity values of 33 % (reciprocal: 21 %), 14 % (reciprocal 16 %) and 36 % (reciprocal 38 %), respectively. Ubiquinone Q-10 was detected as the major respiratory quinone. The predominant fatty acid was Cω7 (71.0 %) and the typical 2-hydroxy fatty acid C 2-OH (11.7 %) was detected. The polar lipid profile contained the diagnostic lipids diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, and ubiquinone, polar lipid and fatty acid composition revealed that strain JM-1 represents a novel species of the genus . For this species we propose the name sp. nov. with the type strain JM-1 ( = LMG 28479 = CCM 8547).

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2015-01-01
2024-04-25
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Proposal of Novosphingobium rhizosphaerae sp. nov., isolated from the rhizosphere
Int J Syst Evol Microbiol 65, 195 (2015); https://doi.org/10.1099/ijs.0.070375-0
/content/journal/ijsem/10.1099/ijs.0.070375-0
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