1887

Abstract

A novel yeast species is described from 19 strains isolated from flowers and insects in three provinces of Cuba. The species is so far known only from Cuba. Characteristic asci and ascospores as well as phylogenetic analysis of the rDNA sequence place the novel species in the genus . The novel species belongs to the New World subclade of large-spored species of . Mating tests with other members of the subclade resulted in the formation of sterile asci without ascospores, showing that the Cuban strains represent a distinct biological species. Intraspecies matings lead to the production of fertile asci containing large needle-shaped ascospores. The novel species was further distinguished from its close relatives by rDNA sequences and PCR fingerprinting using primers derived from mini- and microsatellites. We propose the name sp. nov. and designate MUCL 45753 (=CRGF 279 =CBS 10832, h) as the type strain and MUCL 45751 (=CRGF 278 =CBS 10833, h) as the allotype.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.2008/001198-0
2008-12-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/12/2955.html?itemId=/content/journal/ijsem/10.1099/ijs.0.2008/001198-0&mimeType=html&fmt=ahah

References

  1. Boutroux, M. L.(1884). Conservation des ferments alcooliques dans la nature. Ann Sci Nat Bot 17, 145–209 (in French). [Google Scholar]
  2. Capote, R. P., Berazaín, R. & Leiva, A.(1989). Cuba. In Floristic Inventory of Tropical Countries: The Status of Plant Systematics, Collections, and Vegetation, Plus Recommendations for the Future, pp. 315–335. Edited by D. G. Campbell & D. Hammond. New York: New York Botanical Garden.
  3. Fidalgo-Jiménez, A., Evrard, P., Decock, C. & Daniel, H. M.(2005).Ecological Survey of Yeasts in Cuba. Brasília: Latin-American Congress of Mycology.
  4. Kreger-van Rij, N. J. W.(1987). Classification of yeasts. In The Yeasts, 2nd edn, vol. 1, pp. 5–61. Edited by A. H. Rose & J. S. Harrison. London: Academic Press.
  5. Lachance, M. A., Starmer, W. T., Rosa, C. A., Bowles, J. M., Baker, J. S. F. & Janzen, D. H.(2001). Biogeography of the yeasts of ephemeral flowers and their insects. FEMS Yeast Res 1, 1–8.[CrossRef] [Google Scholar]
  6. Lachance, M. A., Bowles, J. M. & Starmer, W. T.(2003). Geography and niche occupancy as determinants of the yeast biodiversity: the yeast-insect-morning glory ecosystem of Kīpuka Puaulu, Hawai'i. FEMS Yeast Res 4, 105–111.[CrossRef] [Google Scholar]
  7. Lachance, M.-A., Ewing, C. P., Bowles, J. M. & Starmer, W. T.(2005).Metschnikowia hamakuensis sp. nov., Metschnikowia kamakouana sp. nov. and Metschnikowia mauinuiana sp. nov., three endemic yeasts from Hawaiian nitidulid beetles. Int J Syst Evol Microbiol 55, 1369–1377.[CrossRef] [Google Scholar]
  8. Marinoni, G. & Lachance, M. A.(2004). Speciation in the large-spored Metschnikowia clade and establishment of a new species, Metschnikowia borealis comb. nov. FEMS Yeast Res 4, 587–596.[CrossRef] [Google Scholar]
  9. Mena, P. J., Herrera, F. S., Mercado, S. A., Minter, D., Iglesias, B. H., Blanco, H. N., Ortiz, M. J. L., Maldonado, G. S., Recio, H. G. & other authors(2003). Estrategia para la conservación de la diversidad fúngica en Cuba. In Mem. IV Convención Internacional de Medioambiente y Desarrollo, pp. 262–282. Havana: Palacio de las Convenciones (in Spanish).
  10. Miller, M. W. & Phaff, H. J.(1998).Metschnikowia Kamienski. In The Yeasts, a Taxonomic Study, 4th edn, pp. 256–270. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier Science.
  11. Nguyen, N. H., Suh, S. O., Erbil, C. K. & Blackwell, M.(2006).Metschnikowia noctiluminum sp. nov., Metschnikowia corniflorae sp. nov., and Candida chrysomelidarum sp. nov., isolated from green lacewings and beetles. Mycol Res 110, 346–356.[CrossRef] [Google Scholar]
  12. Reukauf, E.(1911–1912). Nektarhefen. Die Kleinwelt 3, 25–26 (in German). [Google Scholar]
  13. Robert, V., Evrard, P. & Hennebert, G. L.(1997). BCCM/ALLEV 2.00 an automated system for the identification of yeasts. Mycotaxon 64, 455–463. [Google Scholar]
  14. Rosa, C. A., Lachance, M.-A., Teixeira, L. C. R. S., Pimenta, R. S. & Morais, P. B.(2007).Metschnikowia cerradonensis sp. nov., a yeast species isolated from ephemeral flowers and their nitidulid beetles in Brazil. Int J Syst Evol Microbiol 57, 161–165.[CrossRef] [Google Scholar]
  15. Van der Walt, J. P. & Yarrow, D.(1984). Methods for the isolation, maintenance, classification and identification of yeasts. In The Yeasts, a Taxonomic Study, pp. 45–104. Edited by N. J. W. Kreger-van Rij. London: Elsevier.
  16. Vassart, G., Georges, M., Monsieur, R., Brocas, H., Lequarre, A. S. & Cristophe, D.(1987). A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235, 683–684.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.2008/001198-0
Loading
/content/journal/ijsem/10.1099/ijs.0.2008/001198-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp. 2955 - 2961

Further details on strains of sp. nov. included in this study.

Results of mating tests after 72 h on GYA at 18 °C.

[PDF file of Supplementary Tables](30 KB)



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error