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Volume 58, Issue 11

sp. nov., isolated from soil Free

Abstract

A Gram-positive, short, rod-shaped, cream-coloured actinobacterium, designated MSL-04, was isolated from a soil sample collected from an agricultural field on Bigeum Island (Republic of Korea) and was subjected to polyphasic characterization to unravel its taxonomic position. The optimum growth temperature and pH were 28–30 °C and pH 7.2–7.4. Chemotaxonomic characteristics such as B2-type peptidoglycan with glycolyl residues, MK-11 and MK-12 as the major menaquinones and a fatty acid profile containing iso- and anteiso-branched fatty acids placed this organism within the genus . Phylogenetic analysis based on 16S rRNA gene sequences confirmed the position of strain MSL-04 in the genus and showed that it belonged to the same clade as but was distinct from its nearest neighbours. The 16S rRNA gene sequence of strain MSL-04 showed the highest levels of similarity with respect to LMG 20991 (97.5 % similarity) and IFO 3750 (97 %). The G+C content of the DNA was 71.0 mol%. The DNA–DNA relatedness between MSL-04 and its closest neighbours (<70 %) showed that the strain represents a distinct genomic species. On the basis of the phenotypic and genotypic results, strain MSL-04 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is MSL-04 (=KCTC 19269 =DSM 19265).

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2008-11-01
2024-04-20
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Microbacterium kribbense sp. nov., isolated from soil
Int J Syst Evol Microbiol 58, 2536 (2008); https://doi.org/10.1099/ijs.0.2008/001222-0
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