@article{mbs:/content/journal/ijsem/10.1099/ijs.0.2008/001602-0, author = "Gutiérrez, M. C. and Castillo, A. M. and Kamekura, M. and Ventosa, A.", title = "Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "12", pages = "2880-2884", doi = "https://doi.org/10.1099/ijs.0.2008/001602-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.2008/001602-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel extremely halophilic strain, designated XH-65T, isolated from the salt lake Xilinhot in Inner Mongolia, PR China, was subjected to a polyphasic taxonomic characterization. Strain XH-65T is neutrophilic, non-motile and requires at least 2.5 M NaCl for growth, with an optimum at 3.4 M NaCl, and grows at pH 6.0–9.0, with optimum growth at pH 7.5. Strain XH-65T grows at 25–50 °C, with optimal growth at 37 °C. Magnesium is not required for growth. On the basis of 16S rRNA gene sequence analysis, strain XH-65T was shown to belong to the genus Haloterrigena and was related to Haloterrigena turkmenica VKM B-1734T (98.1 % sequence similarity), Haloterrigena saccharevitans AB14T (96.9 %), Haloterrigena thermotolerans PR5T (96.3 %), Haloterrigena limicola AX-7T (95.8 %) and Haloterrigena hispanica FP1T (95.7 %). DNA–DNA hybridization revealed 37 % relatedness between strain XH-65T and Htg. turkmenica VKM B-1734T. The polar lipid composition revealed the presence of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1→2)-glucose glycerol diether (S2-DGD). The results of the DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain XH-65T from the six Haloterrigena species with validly published names. Therefore, strain XH-65T represents a novel species, for which the name Haloterrigena salina sp. nov. is proposed, with the type strain XH-65T (=CGMCC 1.6203T =JCM 13891T).", }