1887

Abstract

A bacterial strain, designated B13, was isolated from spoiled red wine from the Dão region, Portugal. The strain was Gram-negative, strictly aerobic, rod-shaped and motile. Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that B13 belonged to the genus within the . The closest related species was , with 98·4 % 16S rRNA gene sequence similarity. DNA–DNA hybridization showed that B13 constituted a taxon separate from the species with validly published names. The DNA G+C content of B13 was 58·1 mol%. Phenotypic characteristics of B13 allowed its differentiation from the recognized species. B13 produced 5-ketogluconic acid from glucose, but no 2-ketogluconic acid. It produced catalase, but no oxidase. It utilized glycerol, but not maltose, ethanol or methanol as carbon sources. On the basis of the results obtained, B13 represents a novel species for which the name sp. nov. is proposed. The type strain is B13 (=LMG 21952=CECT 5830).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.46000-0
2006-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/21.html?itemId=/content/journal/ijsem/10.1099/ijs.0.46000-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  2. Cleenwerck, I., Vandemeulebroecke, K., Janssens, D. & Swings, J.(2002). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52, 1551–1558.[CrossRef] [Google Scholar]
  3. Doetsch, R. N.(1981). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerdhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K.(1998). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef] [Google Scholar]
  7. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  8. Lisdiyanti, P., Kawasaki, H., Seki, T., Yamada, Y., Uchimura, T. & Komagata, K.(2000). Systematic study of the genus Acetobacter with descriptions of Acetobacter indonesiensis sp. nov., Acetobacter tropicalis sp. nov., Acetobacter orleanensis (Henneberg 1906) comb. nov., Acetobacter lovaniensis (Frateur 1950) comb. nov., and Acetobacter estunensis (Carr 1958) comb. nov. J Gen Appl Microbiol 46, 147–165.[CrossRef] [Google Scholar]
  9. Lisdiyanti, P., Kawasaki, H., Seki, T., Yamada, Y., Uchimura, T. & Komagata, K.(2001). Identification of Acetobacter strains isolated from Indonesian sources, and proposals of Acetobacter syzygii sp. nov., Acetobacter cibinongensis sp. nov., and Acetobacter orientalis sp. nov. J Gen Appl Microbiol 47, 119–131.[CrossRef] [Google Scholar]
  10. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  11. Rivas, R., Velázquez, E., Valverde, A., Mateos, P. F. & Martínez-Molina, E.(2001). A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species. Electrophoresis 22, 1086–1089.[CrossRef] [Google Scholar]
  12. Rivas, R., Willems, A., Subba-Rao, N. S., Mateos, P. F., Kroppenstedt, R., Martínez-Molina, E., Gillis, M. & Velázquez, E.(2003). Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India. Syst Appl Microbiol 26, 47–53.[CrossRef] [Google Scholar]
  13. Saitou, N. & Nei, M.(1987). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  14. Silva, L. R., Andrade, P. B., Valentão, P., Seabra, R. M., Trujillo, M. E. & Velázquez, E.(2004). Analysis of non-coloured phenolics in red wine: effect of Dekkera bruxellensis yeast. Food Chem 89, 185–189. [Google Scholar]
  15. Sokollek, S. J., Hertel, C. & Hammes, W. P.(1998). Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations. Int J Syst Bacteriol 48, 935–940.[CrossRef] [Google Scholar]
  16. Wilson, K.(1987). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.46000-0
Loading
/content/journal/ijsem/10.1099/ijs.0.46000-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error