1887

Abstract

The previously proposed species and appear to be similar from their published descriptions. Accordingly, the aim of the current study was to perform phenotypic and genetic analyses of the type strains of both species, in order to clarify their taxonomic positions. The type strains of (DSM 5675) and (NCTC 11158) exhibited identical biochemical profiles and their 16S rRNA gene sequences displayed 99·9 % similarity. DNA–DNA hybridization was also estimated to be 96·5 %. Thus, it was concluded that and are synonyms, where has priority. An emended description of the genus is also given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63068-0
2004-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540947.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63068-0&mimeType=html&fmt=ahah

References

  1. Cato E. P., Hash D. E., Holdeman L. V., Moore W. E. C. 1982; Electrophoretic study of Clostridium species. J Clin Microbiol 15:688–702
    [Google Scholar]
  2. Collins M. D., Shah H. N. 1986; Reclassification of Bacteroides praeacutus Tissier (Holdeman and Moore) in a new genus, Tissierella , as Tissierella praeacuta comb. nov. Int J Syst Bacteriol 36:461–463 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayźabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Farrow J. A. E., Lawson P. A., Hippe H., Gauglitz U., Collins M. D. 1995; Phylogenetic evidence that the gram-negative nonsporulating bacterium Tissierella ( Bacteroides ) praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and description of Tissierella creatinini sp. nov. Int J Syst Bacteriol 45:436–440 [CrossRef]
    [Google Scholar]
  6. Harms C., Schleicher A., Collins M. D., Andreesen J. R. 1998; Tissierella creatinophila sp. nov., a Gram-positive, anaerobic, non-spore-forming, creatinine-fermenting organism. Int J Syst Bacteriol 48:983–993 [CrossRef]
    [Google Scholar]
  7. Holdeman L. V., Cato E. P., Moore W. E. C. 1991 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  8. MacLennan J. D. 1939; The non-saccharolytic plectridial anaerobes. J Pathol Bacteriol 49:535–548 [CrossRef]
    [Google Scholar]
  9. Niel P., Rimbault A., Campion G., Leluan G. 1989; Phenotypic differentiation between Clostridium hastiforme and Clostridium subterminale by headspace gas chromatography. Int J Syst Bacteriol 39:491–492 [CrossRef]
    [Google Scholar]
  10. Powers E. M. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758
    [Google Scholar]
  11. Suen J. C., Hatheway C. L., Steigerwalt A. G., Brenner D. J. 1988; Clostridium argentinense sp. nov.: a genetically homogeneous group composed of all strains of Clostridium botulinum toxin type G and some nontoxigenic strains previously identified as Clostridium subterminale or Clostridium hastiforme . Int J Syst Bacteriol 38:375–381 [CrossRef]
    [Google Scholar]
  12. Suzuki K., Komagata K. 1983; Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int J Syst Bacteriol 33:188–200 [CrossRef]
    [Google Scholar]
  13. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  14. Tissier H. 1908; Recherches sur la flore intestinale normale des enfants âgés d'un à cinq ans. Ann Inst Pasteur (Paris) 22:189–208 (in French
    [Google Scholar]
  15. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63068-0
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error