1887

Abstract

Four strains of Gram-negative, aerobic, psychrotolerant, non-motile, non-pigmented bacteria were isolated from coastal sea-ice and sediment samples. These strains displayed the general chemotaxonomic and phenotypic features of members of the genus . 16S rRNA gene sequencing positioned the three isolates KMM 3646, KMM 3643 and KMM 3645 and isolate KMM 3659 in two distinct lineages within the genus , displaying less than 98·5 % sequence similarity to the type strains of other species. Genomic distinctness was supported by phenotypic differences in growth temperatures, salinity range for growth and metabolic properties. Based on a combination of phenotypic and biochemical characteristics and phylogenetic position, it is proposed that the members of these two distinct lineages represent two novel species, for which the names sp. nov. (type strain Pi2-20=KMM 3646=DSM 15387) and sp. nov. (type strain R7=KMM 3659=DSM 15389) are proposed.

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2004-09-01
2024-03-28
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References

  1. Bowman J. P., Cavanagh J., Austin J. J., Sanderson K. 1996; Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46:841–848 [CrossRef]
    [Google Scholar]
  2. Bowman J. P., McCammon S. A., Brown M. V., Nichols D. S., McMeekin T. A. 1997a; Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63:3068–3078
    [Google Scholar]
  3. Bowman J. P., Nichols D. S., McMeekin T. A. 1997b; Psychrobacter glacincola sp. nov., a halotolerant, psychrophilic bacterium isolated from Antarctic sea ice. Syst Appl Microbiol 20:209–215 [CrossRef]
    [Google Scholar]
  4. Bozal N., Montes M. J., Tudela E., Guinea J. 2003; Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp.nov. and Psychrobacter fozii sp. nov. Int J Syst Evol Microbiol 53:1093–1100 [CrossRef]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:143–153 [CrossRef]
    [Google Scholar]
  6. Denner E. B. M., Mark B., Busse H.-J., Turkiewicz M., Lubitz W. 2001; Psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease. Syst Appl Microbiol 24:44–53 [CrossRef]
    [Google Scholar]
  7. DeSoete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1993 phylip (phylogenetic inference package) version 3.5.1 Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  10. Juni E., Heym G. A. 1986; Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of Gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36:388–391 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Albrecht A., Buczolits S., Busse H.-J. 2002; Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces. Syst Appl Microbiol 25:31–36 [CrossRef]
    [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  14. Maruyama A., Honda D., Yamamoto H., Kitamura K., Higashihara T. 2000; Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench, including a description of the deep-sea species Psychrobacter pacificensis sp. nov. Int J Syst Evol Microbiol 50:835–846 [CrossRef]
    [Google Scholar]
  15. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
    [Google Scholar]
  16. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  17. Romanenko L. A., Schumann P., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2002; Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov., psychrophilic halophiles from marine environments. Int J Syst Evol Microbiol 52:1291–1297 [CrossRef]
    [Google Scholar]
  18. Romanenko L. A., Zhukova N. V., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2003; Assignment of ‘ Alteromonas marinoglutinosa ’ NCIMB 1770 to Pseudoalteromonas mariniglutinosa sp. nov., nom. rev., comb. nov.. Int J Syst Evol Microbiol 531105–1109 [CrossRef]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  22. Wink J. M., Kroppenstedt R. M., Ganguli B. N., Nadkarni S. R., Schumann P., Seibert G., Stackebrandt E. 2003; Three new antibiotic producing species of the genus Amycolatopsis , Amycolatopsis balhimycina sp.nov., A. tolypomycina sp. nov., A. vancoresmycina sp.nov., and description of Amycolatopsis keratiniphila sub.keratiniphila subsp. nov. and A.keratiniphila subsp. nogabecina subsp. nov. Syst Appl Microbiol 26:38–46 [CrossRef]
    [Google Scholar]
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