1887

Abstract

A novel sulphate-reducing bacterium (Al1) was recovered from a soured oil well in Purdu Bay, Alaska. Light and atomic force microscopy observations revealed that cells were Gram-negative, vibrio-shaped and motile by means of a single polar flagellum. The carbon and energy sources used by the isolate and the salinity, temperature and pH ranges facilitating its growth proved to be typical of a partial lactate-oxidizing, moderately halophilic, mesophilic, sulphate-reducing bacterium. Analysis of the fatty acid profile revealed that C, isoC and isoC 7 were the predominant species. Fatty acid profile and complete 16S rRNA gene sequencing demonstrated the similarity between strain Al1 and members of the genus . The position of strain Al1 within the phylogenetic tree indicated that it clustered closely with DSM 10520 (98·9 % sequence similarity), a strain recovered from a similar habitat. However, whole-cell protein profiles, Fourier-transform infrared studies and DNA–DNA hybridization demonstrated that, in spite of the high level of 16S rRNA gene sequence similarity, there is sufficient dissimilarity at the DNA sequence level between DSM 10520 and strain Al1 (10·2 % similarity) to propose that strain Al1 belongs to a separate species within the genus . Based on the results obtained, the name sp. nov. is therefore proposed, with Al1 (=NCIMB 13491=DSM 16109) as the type strain.

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2004-09-01
2024-03-28
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