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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 54, Issue 5

sp. nov., a novel halophilic actinomycete isolated from saline soil in China Free

Abstract

A moderately halophilic actinomycete strain, designated YIM 90010, was isolated from a soil sample collected from a hypersaline habitat in Xinjiang Province, China, and then investigated using a polyphasic taxonomic approach. The strain produced abundant aerial mycelia and fragmented substrate mycelia on most media tested; the optimum NaCl concentration for growth was 10 % (w/v) and the optimum growth temperature and pH were 28 °C and 7·2, respectively. Chemotaxonomically and phylogenetically, the strain was related to members of the genus . The isolate contained chemotaxonomic markers that were diagnostic for the genus , i.e. -diaminopimelic acid, no diagnostic sugars, and MK-10(H), MK-10(H) and MK-12 as the predominant menaquinones. The major fatty acids were iso- and anteiso-branched acids combined with tuberculostearic acid (Me C), straight-chain saturated fatty acids and unsaturated fatty acids. The G+C content was 73·1 mol%. Phylogenetic analysis confirmed that strain YIM 90010 was a member of the genus and most closely related to (97·6 % similarity) and (98·1 % similarity). It can be differentiated from these species by using phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization results. On the basis of the polyphasic evidence, a novel species, sp. nov., is proposed. The type strain of the species is YIM 90010 (=KCTC 19003=CCTCC AA 204009).

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Keyword(s): ISP, International Streptomyces Project
SGM
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2004-09-01
2024-04-19
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Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from saline soil in China
Int J Syst Evol Microbiol 54, 1805 (2004); https://doi.org/10.1099/ijs.0.63127-0
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