@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63128-0, author = "Jeong, Hyunyoung and Yi, Hana and Sekiguchi, Yuji and Muramatsu, Mizuho and Kamagata, Yoichi and Chun, Jongsik", title = "Clostridium jejuense sp. nov., isolated from soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2004", volume = "54", number = "5", pages = "1465-1468", doi = "https://doi.org/10.1099/ijs.0.63128-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63128-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A strictly anaerobic, mesophilic, endospore-forming bacterium, designated strain HY-35-12T, was isolated from a soil sample in Jeju, Korea. Cells of this isolate were Gram-positive, motile rods that formed oval to spherical terminal spores. Strain HY-35-12T grew optimally at 30 °C, pH 7·0 and 0–0·5 % (w/v) NaCl. The isolate produced pyruvate, lactate, acetate, formate and hydrogen as fermentation end products from glucose. The G+C content of DNA of the isolate was 41 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism formed a monophyletic clade with Clostridium xylanovorans and Clostridium aminovalericum in cluster XIVa of the genus Clostridium. The closest phylogenetic neighbour was C. xylanovorans, with 96·65 % 16S rRNA gene sequence similarity. Several physiological and chemotaxonomic properties were identified that enable strain HY-35-12T to be distinguished from phylogenetically related clostridia. On the basis of polyphasic characteristics, it is proposed that strain HY-35-12T (=IMSNU 40003T=KCTC 5026T=DSM 15929T) represents a novel species, Clostridium jejuense sp. nov.", }