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Volume 54, Issue 6

sp. nov., isolated from abnormally spoiled oriental melon in Korea Free

Abstract

Two Gram-positive bacterial strains, CM2104 and CM2110, isolated from the inner part of abnormally spoiled oriental melon () in Korea, were subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan of strains CM2104 and CM2110 contained -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H). The major fatty acids detected in the two strains were iso-C, C 8 and C 9 or C. The DNA G+C content of the two strains was 73 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strains formed a coherent cluster with a clade comprising two species, and . Strains CM2104 and CM2110 exhibited a 16S rRNA gene sequence similarity value of 99·7 % and a mean DNA–DNA relatedness level of 89 %. Strains CM2104 and CM2110 showed 16S rRNA gene sequence similarity levels of 97·8–98·4 % to the type strains of and . DNA–DNA relatedness between strains CM2104 and CM2110 and the type strains of these two species was 7–11 %. On the basis of the phenotypic and phylogenetic data and genomic distinctiveness, strains CM2104 and CM2110 should be placed within the genus as members of a novel species, for which the name sp. nov. is proposed. The type strain is CM2104 (=KCTC 9987=DSM 16063=JCM 12321).

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Keyword(s): FAME, fatty acid methyl ester and TEM, transmission electron microscopy
SGM
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2004-11-01
2024-04-24
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Janibacter melonis sp. nov., isolated from abnormally spoiled oriental melon in Korea
Int J Syst Evol Microbiol 54, 1975 (2004); https://doi.org/10.1099/ijs.0.63167-0
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