RT Journal Article SR Electronic(1) A1 Banat, Ibrahim M. A1 Marchant, Roger A1 Rahman, Thahira J.YR 2004 T1 Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 54 IS 6 SP 2197 OP 2201 DO https://doi.org/10.1099/ijs.0.63231-0 PB Microbiology Society, SN 1466-5034, AB Several aerobic, motile, rod-shaped, thermophilic, spore-forming Geobacillus bacteria predominantly giving a Gram-positive staining reaction were isolated from a cool soil environment in Northern Ireland and taxonomically investigated. Two isolates, F10 and TfT, showed low 16S rRNA gene sequence similarity to recognized members of the genus Geobacillus. Phylogenetic tree investigation using neighbour-joining, maximum-likelihood and parsimony methods indicated that strains F10 and TfT represent a single novel species, for which the name Geobacillus debilis sp. nov. is proposed, with type strain TfT (=DSM 16016T=NCIMB 13995T) and which belongs to a subgroup of the genus Geobacillus comprising Geobacillus toebii and Geobacillus caldoxylosilyticus. However, G. debilis showed closest affinities to Bacillus pallidus, which we propose should become Geobacillus pallidus comb. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63231-0