1887

Abstract

A facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium, strain YK-1, was isolated from an underground crude-oil storage cavity at Kuji in Iwate, Japan. The cells were motile, curved rods and had a single polar flagellum. Optimum growth occurred in a low-strength salt medium at pH 7·0 and 25 °C. It utilized sulfide, elemental sulfur, thiosulfate and hydrogen as the electron donors and nitrate as the electron acceptor under anaerobic conditions, but it did not use nitrite. Oxygen also served as the electron acceptor under the microaerobic condition (O in the head space 1 %). It did not grow on sugars, organic acids or hydrocarbons as carbon and energy sources. The DNA G+C content of strain YK-1 was 45 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that its closest relative was in the ‘’, albeit with low homology (90 %). On the basis of physiological and phylogenetic data, strain YK-1 should be classified into a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is YK-1 (=JCM 11577=MBIC 06352=ATCC BAA-921).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63243-0
2004-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542297.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63243-0&mimeType=html&fmt=ahah

References

  1. Beveridge T. J., Popkin T. J., Cole R. M. 1994; Electron microscopy. In Methods for General and Molecular Bacteriology pp  42–71 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Corre E., Reysenbach A.-L., Prieur D. 2001; Epsilon-proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. FEMS Microbiol Lett 205:329–335
    [Google Scholar]
  3. Engel A. S., Lee N., Porter M. L., Stern L. A., Bennett P. C., Wagner M. 2003; Filamentous “ Epsilonproteobacteria ” dominate microbial mats from sulfidic cave springs. Appl Environ Microbiol 69:5503–5511 [CrossRef]
    [Google Scholar]
  4. Gevertz D., Telang A. J., Voordouw G., Jenneman G. E. 2000; Isolation and characterization of strains CVO and FWKO B, two novel nitrate-reducing, sulfide-oxidizing bacteria isolated from oil field brine. Appl Environ Microbiol 66:2491–2501 [CrossRef]
    [Google Scholar]
  5. Gutell R. R. 1994; Collection of small subunit (16S- and 16S-like) ribosomal RNA structures: 1994. Nucleic Acids Res 22:3502–3507 [CrossRef]
    [Google Scholar]
  6. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  7. Kodama Y., Watanabe K. 2003; Isolation and characterization of a sulfur-oxidizing chemolithotroph growing on crude oil under anaerobic conditions. Appl Environ Microbiol 69:107–112 [CrossRef]
    [Google Scholar]
  8. Lomans B. P., Maas R., Luderer R., den Camp H. J. M. O., Pol A., van der Drift C., Vogels G. D. 1999; Isolation and characterization of Methanomethylovorans hollandica gen. nov., sp. nov., isolated from freshwater sediment, a methylotrophic methanogen able to grow on dimethyl sulfide and methanethiol. Appl Environ Microbiol 65:3641–3650
    [Google Scholar]
  9. Madrid V. M., Taylor G. T., Scranton M. I., Chistoserdov A. Y. 2001; Phylogenetic diversity of bacterial and archaeal communities in the anoxic zone of the Cariaco Basin. Appl Environ Microbiol 67:1663–1674 [CrossRef]
    [Google Scholar]
  10. Maidak B. L., Cole J. R., Parker C. T. Jr 11 other authors 1999; A new version of the RDP (Ribosomal Database Project. Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Moyer C. L., Dobbs F. C., Karl D. M. 1995; Phylogenetic diversity of the bacterial community from a microbial mat at an active, hydrothermal vent system, Loihi Seamount. Hawaii. Appl Environ Microbiol 61:1555–1562
    [Google Scholar]
  13. Riviere J. W. M., Schmidt K. 1992; The genus Thiovulum . In The Prokaryotes pp  3492–3497 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  16. Timmer-ten Hoor A. 1975; A new type of thiosulfate oxidizing, nitrate reducing microorganism: Thiomicrospira denitrificans sp. nov. Neth J Sea Res 9:344–350 [CrossRef]
    [Google Scholar]
  17. Watanabe K., Watanabe K., Kodama Y., Syutsubo K., Harayama S. 2000; Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities. Appl Environ Microbiol 66:4803–4809 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63243-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63243-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error