1887

Abstract

The evolution and taxonomy of enterohepatic species with flexispira morphology were studied by a polyphasic approach including phenotypic characterization, analysis of 16S rRNA and gene sequences and dot-blot DNA–DNA hybridization of whole genomic DNA. In addition, available phylogenetic data on the HSP60 gene were used in the analysis. The study included 14 Finnish canine and feline flexispira strains, the reference strains of sp. flexispira taxa 2, 3 and 8 and ATCC 51630. Phenotypically, all canine and feline strains were similar to . Analysis of 16S rRNA gene sequences of these strains revealed a similarity of 97–99·5 %. Similarity of nucleotide and amino acid sequences within the studied flexispira group was 97–100 % and 99–100 %, respectively, revealing close relatedness. sequences of had only 64–66 % similarity to the flexispira group. The similarity to was 81·5–82·1 %. High levels of DNA–DNA hybridization between the strains were found in dot-blot tests. Polyphasic analysis of the phenotypic and genotypic characteristics of the Finnish flexispira strains and the reference strains of taxa 2, 3 and 8 showed that they differed from other species and are members of the previously described species . In addition, canine strain F56 differed in all phylogenetic analyses from the group and probably represents a novel species.

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2005-03-01
2024-03-28
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References

  1. Altschul S. F., Bundschuh R., Olsen R., Hwa T. 2001; The estimation of statistical parameters for local alignment score distributions. Nucleic Acids Res 29:351–361 [CrossRef]
    [Google Scholar]
  2. Archer J. R., Romero S., Ritchie A. E., Hamacher M. E., Steiner B. M., Bryner J. H., Schell R. F. 1988; Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J Clin Microbiol 26:101–105
    [Google Scholar]
  3. Bryner J. H. 1988; Flexispira rappini , gen. nov., sp. nov. A motile, urease-producing rod similar to Campylobacter pyloridis . In Campylobacter IV. Proceedings of the Fourth International Workshop on Campylobacter Infections pp  440–442 Edited by Kajser B., Falsen E. Sweden: Göterna Kungälv;
    [Google Scholar]
  4. Bryner J. H., Richie A. E., Pollet L., Kirkbride C. A., Collins J. E. 1987; Experimental infection and abortion of pregnant guinea pigs with a unique spirillum-like bacterium isolated from aborted ovine fetuses. Am J Vet Res 48:91–95
    [Google Scholar]
  5. Dewhirst F. E., Fox J. G., Mendes E. N., Paster B. J., Gates C. E., Kirkbride C. A., Eaton K. A. 2000a; Flexispira rappini ’ strains represent at least 10 Helicobacter taxa. Int J Syst Evol Microbiol 50:1781–1787
    [Google Scholar]
  6. Dewhirst F. E., Fox J. G., On S. L. W. 2000b; Recommended minimal standards for describing new species of the genus Helicobacter . Int J Syst Evol Microbiol 50:2231–2237 [CrossRef]
    [Google Scholar]
  7. Fox J. G., Correa P., Taylor N. S., Lee A., Otto G., Murphy J. C., Rose R. 1990; Helicobacter mustelae -associated gastritis in ferrets. An animal model of Helicobacter pylori gastritis in humans. Gastroenterology 99:352–361
    [Google Scholar]
  8. Fox J. G., Yan L. L., Dewhirst F. E., Paster B. J., Shames B., Murphy J. C., Hayward A., Belcher J. C., Mendes E. N. 1995; Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice. J Clin Microbiol 33:445–454
    [Google Scholar]
  9. Fox J. G., Dewhirst F., Shen Z. 8 other authors 1998; Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology 114:755–763 [CrossRef]
    [Google Scholar]
  10. Fox J. G., Shen Z., Feng Y., Dangler C. A., Dewhirst F. E., Paster B. J., Cullen C. M. 2002; Helicobacter marmotae sp. nov. isolated from livers of woodchucks and intestines of cats. J Clin Microbiol 40:2513–2519 [CrossRef]
    [Google Scholar]
  11. Gueneau P., Loiseaux-De Goër S. 2002; Helicobacter : molecular phylogeny and the origin of gastric colonization in the genus. Infect Genet Evol 1:215–223 [CrossRef]
    [Google Scholar]
  12. Hänninen M. L., Happonen I., Saari S., Jalava K. 1996; Culture and characteristics of Helicobacter bizzozeronii , a new canine gastric Helicobacter sp. Int J Syst Bacteriol 46:160–166 [CrossRef]
    [Google Scholar]
  13. Hänninen M. L., Utriainen M., Happonen I., Dewhirst F. E. 2003; Helicobacter sp. flexispira 16S rDNA taxa 1, 4 and 5 and Finnish porcine Helicobacter isolates are members of the species Helicobacter trogontum (taxon 6). Int J Syst Evol Microbiol 53:425–433 [CrossRef]
    [Google Scholar]
  14. Hurtado A., Clewley J. P., Linton D., Owen R. J., Stanley J. 1997; Sequence similarities between large subunit ribosomal RNA gene intervening sequences from different Helicobacter species. Gene 194:69–75 [CrossRef]
    [Google Scholar]
  15. Iten A., Graf S., Egger M., Täuber M., Graf J. 2001; Helicobacter sp. flexispira bacteremia in an immunocompetent young adult. J Clin Microbiol 39:1716–1720 [CrossRef]
    [Google Scholar]
  16. Jalava K., Kaartinen M., Utriainen M., Happonen I., Hänninen M. L. 1997; Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii . Int J Syst Bacteriol 47:975–982 [CrossRef]
    [Google Scholar]
  17. Jalava K., On S. L. W., Vandamme P. A., Happonen I., Sukura A., Hänninen M.-L. 1998; Isolation and identification of Helicobacter spp. from canine and feline gastric mucosa. Appl Environ Microbiol 64:3998–4006
    [Google Scholar]
  18. Jalava K., On S., Harrington C. S., Andersen L., Hänninen M.-L., Vandamme P. 2001; A cultured strain of “ Helicobacter heilmannii ,” a human gastric pathogen, identified as H. bizzozeronii : evidence for zoonotic potential of Helicobacter . Emerg Infect Dis 7:1036–1038 [CrossRef]
    [Google Scholar]
  19. Kirkbride C. A., Gates C. E., Collins J. E. 1986; Abortion in sheep caused by a nonclassified, anaerobic, flagellated bacterium. Am J Vet Res 47:259–262
    [Google Scholar]
  20. Kostia S., Veijalainen P., Hirvi U., Hänninen M.-L. 2003; Cytolethal distending toxin B gene ( cdtB ) homologues in taxa 2, 3 and 8 and in six canine isolates of Helicobacter sp. flexispira. J Med Microbiol 52:103–108 [CrossRef]
    [Google Scholar]
  21. Lee A., Phillips M. W. O'Rourke., L J. 7 other authors 1992; Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int J Syst Bacteriol 42:27–36 [CrossRef]
    [Google Scholar]
  22. Linton D., Clewley J. P., Burnens A., Owen R. J., Stanley J. 1994; An intervening sequence (IVS) in the 16S rRNA gene of the eubacterium Helicobacter canis . Nucleic Acids Res 22:1954–1958 [CrossRef]
    [Google Scholar]
  23. Ljungh Å., Wadström T. 2002; The role of microorganisms in biliary tract disease. Curr Gastroenterol Rep 4:167–171 [CrossRef]
    [Google Scholar]
  24. Matsukura N., Yokomuro S., Yamada S., Tajiri T., Sundo T., Hadama T., Kamiya S., Naito Z., Fox J. G. 2002; Association between Helicobacter bilis in bile and biliary tract malignancies: H. zbilis in bile from Japanese and Thai patients with benign and malignant diseases in the biliary tract. Jpn J Cancer Res 93:842–847 [CrossRef]
    [Google Scholar]
  25. Mendes E. N., Queiroz D. M., Dewhirst F. E., Paster B. J., Moura S. B., Fox J. G. 1996; Helicobacter trogontum sp. nov., isolated from the rat intestine. Int J Syst Bacteriol 46:916–921 [CrossRef]
    [Google Scholar]
  26. Mikkonen T., Kärenlampi R., Hänninen M.-L. 2004; Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences. Int J Syst Evol Microbiol 54:753–758 [CrossRef]
    [Google Scholar]
  27. Nilsson I., Kornilovs'ka I., Lindgren S., Ljungh Å., Wadström T. 2003; Increased prevalence of seropositivity for non-gastric Helicobacter species in patients with autoimmune liver disease. J Med Microbiol 52:949–953 [CrossRef]
    [Google Scholar]
  28. Paster B. J., Lee A., Fox J. G., Dewhirst F. E., Tordoff L. A., Fraser G. J., O'Rourke J. L., Taylor N. S., Ferrero R. 1991; Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae , and related bacteria. Int J Syst Bacteriol 41:31–38 [CrossRef]
    [Google Scholar]
  29. Patterson M. M., Schrenzel M. D., Feng Y., Xu S., Dewhirst F. E., Paster B. J., Thibodeau S. A., Versalovic J., Fox J. G. 2000; Helicobacter aurati sp. nov., a urease-positive Helicobacter species cultured from gastrointestinal tissues of Syrian hamsters. J Clin Microbiol 38:3722–3728
    [Google Scholar]
  30. Robertson B. R., O'Rourke J. L., Vandamme P., On S. L., Lee A. 2001; Helicobacter ganmani sp. nov., a urease-negative anaerobe isolated from the intestines of laboratory mice. Int J Syst Evol Microbiol 51:1881–1889 [CrossRef]
    [Google Scholar]
  31. Romero S., Archer J. R., Hamacher M. E., Bologna S. M., Schell R. F. 1988; Case report of an unclassified microaerophilic bacterium associated with gastroenteritis. J Clin Microbiol 26:142–143
    [Google Scholar]
  32. Saunders K. E., Shen Z., Dewhirst F. E., Paster B. J., Dangler C. A., Fox J. G. 1999; Novel intestinal Helicobacter species isolated from cotton-top tamarins ( Saguinus oedipus ) with chronic colitis. J Clin Microbiol 37:146–151
    [Google Scholar]
  33. Shen Z., Fox J. G., Dewhirst F. E., Paster B. J., Foltz C. J., Yan L., Shames B., Perry L. 1997; Helicobacter rodentium sp. nov., a urease-negative Helicobacter species isolated from laboratory mice. Int J Syst Bacteriol 47:627–634 [CrossRef]
    [Google Scholar]
  34. Silva C. P., Pereira-Lima J. C., Oliveira A. G., Guerra J. B., Marques D. J., Sarmanho L., Cabral M. M. D. Á., Queiroz D. M. M. 2003; Association of the presence of Helicobacter in gallbladder tissue with cholelithiasis and cholecystitis. J Clin Microbiol 41:5615–5618 [CrossRef]
    [Google Scholar]
  35. Solnick J. V., Schauer D. B. 2001; Emergence of diverse Helicobacter species in the pathogenesis of gastric and enterohepatic diseases. Clin Microbiol Rev 14:59–97 [CrossRef]
    [Google Scholar]
  36. Sorlin P., Vandamme P., Nortier J., Hoste B., Rossi C., Pavlof S., Struelens M. J. 1999; Recurrent “ Flexispira rappini ” bacteremia in an adult patient undergoing hemodialysis: case report. J Clin Microbiol 37:1319–1323
    [Google Scholar]
  37. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  38. Stanley J., Linton D., Burnens A. P., Dewhirst F. E., On S. L., Porter A., Owen R. J., Costas M. 1994; Helicobacter pullorum sp. nov. – genotype and phenotype of a new species isolated from poultry and from human patients with gastroenteritis. Microbiology 140:3441–3449 [CrossRef]
    [Google Scholar]
  39. Tee W., Leder K., Karroum E., Dyall-Smith M. 1998; Flexispira rappini ” bacteremia in a child with pneumonia. J Clin Microbiol 36:1679–1682
    [Google Scholar]
  40. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60:407–438
    [Google Scholar]
  41. Vandamme P., Harrington C. S., Jalava K., On S. L. 2000; Misidentifying helicobacters: the Helicobacter cinaedi example. J Clin Microbiol 38:2261–2266
    [Google Scholar]
  42. Weir S., Cuccherini B., Whitney A. M. 9 other authors 1999; Recurrent bacteremia caused by a “ Flexispira ”-like organism in a patient with X-linked (Bruton's) agammaglobulinemia. J Clin Microbiol 37:2439–2445
    [Google Scholar]
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