1887

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Volume 55, Issue 2

Benthic cyanobacteria of the genus are non-toxic, without gas vacuoles, able to glide and genetically more diverse than planktonic Free

Abstract

Diversity and ecological features of cyanobacteria of the genus from benthic, periphytic and soil habitats are less well known than those of from planktonic habitats. Novel benthic strains were isolated from the Baltic Sea and their morphology, the presence of gas vacuoles, nodularin production, gliding, 16S rRNA gene sequences, , and genes, and -IGS regions were examined, as well as short tandemly repeated repetitive sequence fingerprints. Strains were identified as , or on the basis of the size and shape of the different types of cells and the presence or absence of gas vacuoles. The planktonic strains of mostly had gas vacuoles and produced nodularin, whereas the benthic strains of and lacked gas vacuoles and did not produce nodularin (except for strain PCC 7804). The benthic strains were also able to glide on surfaces. In the genetic analyses, the planktonic and benthic formed monophyletic clusters, but the clusters were very closely related. Benthic strains determined as formed the most diverse and distant group of strains. In addition to phylogenetic analyses, the lack of the -IGS region and in and distinguished these species from the planktonic . Therefore, can be considered as a potential diagnostic tool for detecting and quantifying Baltic Sea bloom-forming, nodularin-producing strains. The data confirm that only one morphologically and genetically distinct planktonic species of , , and at least two benthic species, and , exist in the Baltic Sea.

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Keyword(s): gvpA-IGS, intergenic spacer between two copies of gas vacuole protein A genes , ITS, internal transcribed spacer , mcyE, microcystin synthetase subunit E gene , ML, maximum-likelihood , MP, maximum-parsimony , ndaF, nodularin synthetase subunit F gene , NJ, neighbour-joining , PC-IGS, phycocyanin operon intergenic spacer , PCA, principal component analysis , PP1, protein phosphatase 1 , rbcLX, ribulose-1,5-bisphosphate carboxylase/oxygenase subunit L and chaperonin-like protein X genes , rpoB, DNA-dependent RNA polymerase subunit B gene and STRR sequence, short tandemly repeated repetitive sequence
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2005-03-01
2024-04-18
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Benthic cyanobacteria of the genus Nodularia are non-toxic, without gas vacuoles, able to glide and genetically more diverse than planktonic Nodularia
Int J Syst Evol Microbiol 55, 555 (2005); https://doi.org/10.1099/ijs.0.63288-0
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