@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63356-0, author = "Spring, Stefan and Wagner, Michael and Schumann, Peter and Kämpfer, Peter", title = "Malikia granosa gen. nov., sp. nov., a novel polyhydroxyalkanoate- and polyphosphate-accumulating bacterium isolated from activated sludge, and reclassification of Pseudomonas spinosa as Malikia spinosa comb. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2005", volume = "55", number = "2", pages = "621-629", doi = "https://doi.org/10.1099/ijs.0.63356-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63356-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "PHA, polyhydroxyalkanoate", keywords = "poly-P, polyphosphate", keywords = "EBPR, enhanced biological phosphorus removal", keywords = "PAO, phosphate-accumulating organism", abstract = "A Gram-negative, motile, rod-shaped bacterium, designated strain P1T, was isolated from activated sludge of a municipal wastewater treatment plant. Phylogenetic analysis of its 16S rRNA gene sequence placed the novel isolate among representatives of the family Comamonadaceae. The closest relatives in reconstructed phylogenetic trees were Pseudomonas spinosa, Macromonas bipunctata and Hydrogenophaga species. Strain P1T was not able to grow anaerobically or autotrophically, reduced nitrate to nitrite and required vitamins for growth. Ubiquinone 8 (Q8) and 3-hydroxy-substituted fatty acids were present, but 2-hydroxy fatty acids were absent. The G+C content of the DNA was 67 mol%. Phenotypic characteristics allowed a clear differentiation of strain P1T from representatives of the genera Hydrogenophaga and Macromonas, whereas DNA–DNA hybridization experiments revealed that strain P1T did not belong to the species P. spinosa. As a peculiarity, cells of strain P1T and P. spinosa ATCC 14606T were able to accumulate large amounts of polyhydroxyalkanoates and polyphosphate in the form of large intracellular granules. Apparently in both strains nitrogen limitation stimulates the production of polyhydroxyalkanoates, whereas carbon starvation induces the formation of polyphosphates. Based upon phylogenetic and phenotypic evidence, it is proposed to establish the novel taxon Malikia granosa gen. nov., sp. nov., represented by the type strain P1T (=DSM 15619T=JCM 12706T=CIP 108194T). The most closely related species of strain P1T was P. spinosa. This species has been misclassified, and it is proposed to transfer it to the new genus Malikia as Malikia spinosa gen. nov., comb. nov. The type strain is ATCC 14606T (=DSM 15801T).", }