1887

Abstract

Limit-dilution procedures were used to isolate seven, helically coiled bacterial strains from faeces of swine that constituted two unidentified taxa. Comparative 16S rRNA gene sequence analysis showed highest similarity values with species of the genus indicating that the isolates are members of this genus. Strain 7CPL208, as well as five further isolates, and 14V28 displayed the highest 16S rRNA gene sequence similarities with ATCC 33768 (92·3 %) and OMZ 833 (89·9 %), respectively. Polar lipid profiles distinguished 7CPL208 and 14V28 from each other as well as from related species. Based on their phenotypic and genotypic distinctiveness, strains 7CPL208 and 14V28 are suggested to represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strain for is 7CPL208 (=ATCC BAA-909=CIP 108244=JCM 12341) and for 14V28 (=ATCC BAA-908=CIP 108245=JCM 12342).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63388-0
2005-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/4/ijs551675.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63388-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Akimov V. N., Lubitz W., Busse H.-J. 1997; Polyamine distribution in actinomycetes with Group B peptidoglycan and species of the genera Brevibacterium , Corynebacterium , and Tsukamurella . Int J Syst Bacteriol 47:270–277 [CrossRef]
    [Google Scholar]
  2. Cwyk W. M., Canale-Parola E. 1979; Treponema succinifaciens sp. nov., an anaerobic spirochete from the swine intestine. Arch Microbiol 122:231–239 [CrossRef]
    [Google Scholar]
  3. Dewhirst F. E., Tamer M. A., Ericson R. E., Lau C. N., Levanos V. A., Boches S. K., Galvin J. L., Paster B. J. 2000; The diversity of periodontal spirochetes by 16S rRNA analysis. Oral Microbiol Immunol 15:196–202 [CrossRef]
    [Google Scholar]
  4. Graber J. R., Leadbetter J. R., Breznak J. A. 2004; Description of Treponema azotonutricium sp.nov. and Treponema primitia sp. nov., the first spirochetes isolated from termite guts. Appl Environ Microbiol 70, 1315–1320 [CrossRef]
    [Google Scholar]
  5. Kimura Y., Takahashi M., Matsumoto N. 8 other authors 1993; Verrucose dermatitis and digital papillomatosis in dairy cows. J Vet Med Jpn 46:899–906
    [Google Scholar]
  6. Lilburn T. G., Schmidt T. M., Breznak J. A. 1999; Phylogenetic diversity of termite gut spirochaetes. Environ Microbiol 1:331–345 [CrossRef]
    [Google Scholar]
  7. Lilburn T. G., Kim K. S., Ostrom N. E., Byzek K. R., Leadbetter J. R., Breznak J. A. 2001; Nitrogen fixation by symbiotic and free-living spirochetes. Science 292:2495–2498 [CrossRef]
    [Google Scholar]
  8. Livermore B. P., Johnson R. C. 1974; Lipids of the Spirochaetales : comparison of the lipids of several members of the genera Spirochaeta, Treponema , and Leptospira . J Bacteriol 120:1268–1273
    [Google Scholar]
  9. Livermore B. P., Johnson R. C. 1975; The lipids of four unusual non-pathogenic host-associated spirochetes. Can J Microbiol 21:1877–1880 [CrossRef]
    [Google Scholar]
  10. Matthews H. M., Yang T.-K., Jenkin H. M. 1979; Unique lipid composition of Treponema pallidum (Nichols virulent strain). Infect Immun 24:713–719
    [Google Scholar]
  11. Paster B. J., Canale-Parola E. 1985; Treponema saccharophilum sp. nov., a large pectinolytic spirochete from the bovine rumen. Appl Environ Microbiol 50:212–219
    [Google Scholar]
  12. Sakamoto M., Takeuchi Y., Umeda M., Ishikawa I., Benno Y., Nakase T. 1999; Detection of Treponema socranskii associated with human periodontitis by PCR. Microbiol Immunol 43:485–490 [CrossRef]
    [Google Scholar]
  13. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Schrank K., Choi B. K., Grund S., Moter A., Heuner K., Nattermann H., Gobel U. B. 1999; Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis. Int J Syst Bacteriol 49:43–50 [CrossRef]
    [Google Scholar]
  15. Siqueira J. F., Rocas I. N. 2003; PCR-based identification of Treponema maltophilum , T. amylovorum , T. medium , and T. lecithinolyticum in primary root canal infections. Arch Oral Biol 48:495–502 [CrossRef]
    [Google Scholar]
  16. Smibert R. M., Burmeister J. A. 1983; Treponema pectinovorum sp. nov. isolated from humans with periodontitis. Int J Syst Bacteriol 33:852–856 [CrossRef]
    [Google Scholar]
  17. Smibert R. M., Claterbaugh R. L. Jr 1972; A chemically defined medium for Treponema strain PR-7 isolated from the intestine of a pig with swine dysentery. Can J Microbiol 18:1073–1078 [CrossRef]
    [Google Scholar]
  18. Stanton T. B., Canale-Parola E. 1980; Treponema bryantii sp. nov., a rumen spirochete that interacts with cellulolytic bacteria. Arch Microbiol 127:145–156 [CrossRef]
    [Google Scholar]
  19. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  20. Umemoto T., Nakazawa F., Hoshino E., Okada K., Fukunaga M., Namikawa I. 1997; Treponema medium sp. nov., isolated from human subgingival dental plaque. Int J Syst Bacteriol 47:67–72 [CrossRef]
    [Google Scholar]
  21. Wyss C. 1992; Growth of Porphyromonas gingivalis , Treponema denticola , T. pectinovorum , T. socranskii , and T. vincentii in a chemically defined medium. J Clin Microbiol 30:2225–2229
    [Google Scholar]
  22. Wyss C., Choi B. K., Schupbach P., Guggenheim B., Gobel U. B. 1996; Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int J Syst Bacteriol 46:745–752 [CrossRef]
    [Google Scholar]
  23. Wyss C., Choi B. K., Schupbach P., Guggenheim B., Gobel U. B. 1997; Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int J Syst Bacteriol 47:842–845 [CrossRef]
    [Google Scholar]
  24. Wyss C., Choi B. K., Schupbach P., Moter A., Guggenheim B., Gobel U. B. 1999; Treponema lecithinolyticum sp. nov., a small saccharolytic spirochaete with phospholipase A and C activities associated with periodontal diseases. Int J Syst Bacteriol 49:1329–1339 [CrossRef]
    [Google Scholar]
  25. Wyss C., Dewhirst F. E., Gmur R., Thurnheer T., Xue Y., Schupbach P., Guggenheim B., Paster B. J. 2001; Treponema parvum sp. nov., a small, glucuronic or galacturonic acid-dependent oral spirochaete from lesions of human periodontitis and acute necrotizing ulcerative gingivitis. Int J Syst Evol Microbiol 51:955–962 [CrossRef]
    [Google Scholar]
  26. Wyss C., Moter A., Choi B. K., Dewhirst F. E., Xue Y., Schupbach P., Gobel U. B., Paster B. J., Guggenheim B. 2004; Treponema putidum sp. nov., a medium-sized proteolytic spirochaete isolated from lesions of human periodontitis and acute necrotizing ulcerative gingivitis. Int J Syst Evol Microbiol 54:1117–1122 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63388-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63388-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error