RT Journal Article SR Electronic(1) A1 Wu, Sue-Yao A1 Chen, Sheng-Chung A1 Lai, Mei-ChinYR 2005 T1 Methanofollis formosanus sp. nov., isolated from a fish pond JF International Journal of Systematic and Evolutionary Microbiology, VO 55 IS 2 SP 837 OP 842 DO https://doi.org/10.1099/ijs.0.63475-0 PB Microbiology Society, SN 1466-5034, AB A mesophilic, hydrogenotrophic methanogen, strain ML15T, was isolated from an aquaculture fish pond near Wang-gong, Taiwan. The cells were irregular cocci, non-motile, 1·5–2·0 μm in diameter and Gram-negative. Cells of strain ML15T lysed easily in the presence of SDS (0·1 g l−1) and the S-layer protein had an M r of 138 800. The catabolic substrates utilized by this strain included formate and H2/CO2, but not acetate, methanol, trimethylamine or secondary alcohols. Growth did not occur in minimal medium, but was observed when yeast extract and tryptone were added. Strain ML15T grew fastest at 37 °C, pH 6·6–7·0 and with 3 % NaCl. Acetate was not required for cell growth. Trace amounts of tungstate promoted cell growth. The G+C contents of DNA of Methanofollis aquaemaris N2F9704T and strain ML15T were 59·1 and 58·4 mol%, respectively. Sequence analysis of the 16S rRNA genes of strain ML15T and selected Methanofollis species revealed similarities of 95–97 %. Based on the data presented here, it is proposed that strain ML15T (=OCM 789T=DSM 15483T) represents a novel species, Methanofollis formosanus sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63475-0