1887

Abstract

Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys ( L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and gene sequencing, and from the most closely related species by using DNA–DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the species group (99 % similarity) but are biochemically similar to , from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from -mannitol, the inability to hydrolyse aesculin and DNA and the absence of -glucosidase. On the basis of these analyses, a novel species of the genus is described, for which the name sp. nov. is proposed, with CCM 7213 (=LMG 22723) as the type strain.

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2005-09-01
2024-03-28
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References

  1. Chesneau O., Allignet J., El Solh N. 1993; Thermonuclease gene as a target nucleotide sequence for specific recognition of Staphylococcus aureus . Mol Cell Probes 7:301–310 [CrossRef]
    [Google Scholar]
  2. Chesneau O., Morvan A., Aubert S., El Solh N. 2000; The value of rRNA gene restriction site polymorphism analysis for delineating taxa in the genus Staphylococcus . Int J Syst Evol Microbiol 50:689–697 [CrossRef]
    [Google Scholar]
  3. Couto I., Pereira S., Miragaia M., Santos-Sanches I., de Lencastre H. 2001; Identification of clinical staphylococcal isolates from humans by internal transcribed spacer PCR. J Clin Microbiol 39:3099–3103 [CrossRef]
    [Google Scholar]
  4. Crouch T. W., Higuchi J. H., Coalson J. J., Johanson W. G. Jr 1984; Pathogenesis and prevention of nosocomial pneumonia in a nonhuman primate model of acute respiratory failure. Am Rev Respir Dis 130:502–504
    [Google Scholar]
  5. Devriese L. A., Poutrel B., Kilpper-Bälz R., Schleifer K. H. 1983; Staphylococcus gallinarum and Staphylococcus caprae , two new species from animals. Int J Syst Bacteriol 33:480–486 [CrossRef]
    [Google Scholar]
  6. Dufour P., Jarraud S., Vandenesch F., Greenland T., Novick R. P., Bes M., Etienne J., Lina G. 2002; High genetic variability of the agr locus in Staphylococcus species. J Bacteriol 184:1180–1186 [CrossRef]
    [Google Scholar]
  7. Freney J., Kloos W. E., Hájek V., Webster J. A., Bes M., Brun Y., Vernozy-Rozand C. 1999; Recommended minimal standards for description of new staphylococcal species. Int J Syst Bacteriol 49:489–502 [CrossRef]
    [Google Scholar]
  8. Garrity G. M., Bell J. A., Lilburn T. G. 2004; Taxonomic outline of the Procaryotes. In Bergey's Manual of Systematic Bacteriology , 2nd edn, Release 5.0. New York: Springer; . doi: 10.1007/bergeysoutline200405 http://141.150.157.80/bergeysoutline/main.htm
    [Google Scholar]
  9. George C. G., Kloos W. E. 1994; Comparison of the Sma I-digested chromosomes of Staphylococcus epidermidis and the closely related species Staphylococcus capitis and Staphylococcus caprae . Int J Syst Bacteriol 44:404–409 [CrossRef]
    [Google Scholar]
  10. Goh S. H., Potter S., Wood J. O., Hemmingsen S. M., Reynolds R. P., Chow A. W. 1996; HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol 34:818–823
    [Google Scholar]
  11. Jensen M. A., Webster J. A., Straus N. 1993; Rapid identification of bacteria on the basis of PCR-amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol 59:945–952
    [Google Scholar]
  12. Johnson W. M., Tyler S. D., Ewan E. P., Ashton F. E., Pollard D. R., Rozee K. R. 1991; Detection of genes for enterotoxins, exfoliative toxins, and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction. J Clin Microbiol 29:426–430
    [Google Scholar]
  13. Kawamura Y., Hou X. G., Sultana F., Hirose K., Miyake M., Shu S. E., Ezaki T. 1998; Distribution of Staphylococcus species among human clinical specimens and emended description of Staphylococcus caprae . J Clin Microbiol 36:2038–2042
    [Google Scholar]
  14. Kloos W. E., Bannerman T. L. 1999; Staphylococcus and Micrococcus . In Manual of Clinical Microbiology , 7th edn. pp  264–282 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Kloos W. E., Wolfshohl J. F. 1991; Staphylococcus cohnii subspecies: Staphylococcus cohnii subsp. cohnii subsp. nov. and Staphylococcus cohnii subsp. urealyticum subsp. nov.. Int J Syst Bacteriol 41:284–289 [CrossRef]
    [Google Scholar]
  16. Kloos W. E., Zimmerman R. J., Smith R. F. 1976; Preliminary studies on the characterization and distribution of Staphylococcus and Micrococcus species on animal skin. Appl Environ Microbiol 31:53–59
    [Google Scholar]
  17. Kloos W. E., Schleifer K. H., Götz F. 1992; The genus Staphylococcus . In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn. vol 2 pp  1369–1420 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  18. Kunst F., Ogasawara N., Moszer I. 148 other authors 1997; The complete genome sequence of the Gram-positive bacterium Bacillus subtilis . Nature 390:249–256 [CrossRef]
    [Google Scholar]
  19. Kwok A. Y., Chow A. W. 2003; Phylogenetic study of Staphylococcus and Macrococcus species based on partial hsp60 gene sequences. Int J Syst Evol Microbiol 53:87–92 [CrossRef]
    [Google Scholar]
  20. Mannerová S., Pantůček R., Doškař J., Švec P., Snauwaert C., Vancanneyt M., Swings J., Sedláček I. 2003; Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov.and Macrococcus lamae sp. nov., from the skin of llamas. Int J Syst Evol Microbiol 53:1647–1654 [CrossRef]
    [Google Scholar]
  21. Mendoza M., Meugnier H., Bes M., Etienne J., Freney J. 1998; Identification of Staphylococcus species by 16S–23S rDNA intergenic spacer PCR analysis. Int J Syst Bacteriol 48:1049–1055 [CrossRef]
    [Google Scholar]
  22. Padovan D., Cantrell C. 1983; Causes of death of infant rhesus and squirrel monkeys. J Am Vet Med Assoc 183:1182–1184
    [Google Scholar]
  23. Pantůček R., Götz F., Doškař J., Rosypal S. 1996; Genomic variability of Staphylococcus aureus and the other coagulase-positive Staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis. Int J Syst Bacteriol 46:216–222 [CrossRef]
    [Google Scholar]
  24. Pantůček R., Sedláček I., Doškař J., Rosypal S. 1999; Complex genomic and phenotypic characterization of the related species Staphylococcus carnosus and Staphylococcus piscifermentans . Int J Syst Bacteriol 49:941–951 [CrossRef]
    [Google Scholar]
  25. Place R. B., Hiestand D., Gallmann H. R., Teuber M. 2003; Staphylococcus equorum subsp. linens , subsp. nov., a starter culture component for surface ripened semi-hard cheeses. Syst Appl Microbiol 26:30–37 [CrossRef]
    [Google Scholar]
  26. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  27. Probst A. J., Hertel C., Richter L., Wassill L., Ludwig W., Hammes W. P. 1998; Staphylococcus condimenti sp. nov., from soy sauce mash, and Staphylococcus carnosus (Schleifer and Fischer 1982)subsp. utilis subsp. nov. Int J Syst Bacteriol 48:651–658 [CrossRef]
    [Google Scholar]
  28. Rosenblum L. A., Coe C. L. 1985 Handbook of Squirrel Monkey Research New York: Plenum;
    [Google Scholar]
  29. Schmitz F. J., MacKenzie C. R., Hofmann B., Verhoef J., Finken-Eigen M., Heinz H. P., Kohrer K. 1997; Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by a multiplex PCR. J Med Microbiol 46:773–778 [CrossRef]
    [Google Scholar]
  30. Spergser J., Wieser M., Taubel M., Rossello-Mora R. A., Rosengarten R., Busse H. J. 2003; Staphylococcus nepalensis sp. nov., isolated from goats of the Himalayan region. Int J Syst Evol Microbiol 53:2007–2011 [CrossRef]
    [Google Scholar]
  31. Stasilevich Z. K., Trots A. A., Dzhikidze E. K. 1986; Biotyping of staphylococci isolated from monkeys. Mikrobiol Zh 48:38–41 (in Russia
    [Google Scholar]
  32. Štěpán J., Pantůček R., Růžičková V., Rosypal S., Hájek V., Doškař J. 2001; Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from a common 44-kb Sma I restriction fragment. Mol Cell Probes 15:249–257 [CrossRef]
    [Google Scholar]
  33. Švec P., Devriese L. A., Sedláček I., Baele M., Vancanneyt M., Haesebrouck F., Swings J., Doškař J. 2001; Enterococcus haemoperoxidus sp. nov and Enterococcus moraviensis sp. nov., isolated from water. Int J Syst Evol Microbiol 51:1567–1574
    [Google Scholar]
  34. Švec P., Vancanneyt M., Sedláček I., Engelbeen K., Štetina V., Swings J., Petráš P. 2004; Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwinska et al . 1995 as a later synonym of Staphylococcus vitulinus Webster et al . 1994. Int J Syst Evol Microbiol 54:2213–2215 [CrossRef]
    [Google Scholar]
  35. Takahashi T., Satoh I., Kikuchi N. 1999; Phylogenetic relationships of 38 taxa of the genus Staphylococcus based on 16S rRNA gene sequence analysis. Int J Syst Bacteriol 49:725–728 [CrossRef]
    [Google Scholar]
  36. Tanasupawat S., Hashimoto Y., Ezaki T., Kozaki M., Komagata K. 1991; Identification of Staphylococcus carnosus strains from fermented fish and soy sauce mash. J Gen Appl Microbiol 37:479–494 [CrossRef]
    [Google Scholar]
  37. Tanasupawat S., Hashimoto Y., Ezaki T., Kozaki M., Komagata K. 1992; Staphylococcus piscifermentans sp. nov., from fermented fish in Thailand. Int J Syst Bacteriol 42:577–581 [CrossRef]
    [Google Scholar]
  38. Trülzsch K., Rinder H., Trček J., Bader L., Wilhelm U., Heesemann J. 2002; Staphylococcus pettenkoferi ”, a novel staphylococcal species isolated from clinical specimens. Diagn Microbiol Infect Dis 43:175–182 [CrossRef]
    [Google Scholar]
  39. van Belkum A., Bax R., Peerbooms P., Goessens W. H., van Leeuwen N., Quint W. G. 1993; Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 31:798–803
    [Google Scholar]
  40. Vannuffel P., Gigi J., Ezzedine H., Vandercam B., Delmee M., Wauters G., Gala J.-L. 1995; Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J Clin Microbiol 33:2864–2867
    [Google Scholar]
  41. Versalovic J., Koeuth T., Lupski J. R. 1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823–6831 [CrossRef]
    [Google Scholar]
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