RT Journal Article SR Electronic(1) A1 Pantůček, Roman A1 Sedláček, Ivo A1 Petráš, Petr A1 Koukalová, Dagmar A1 Švec, Pavel A1 Štětina, Vlastimil A1 Vancanneyt, Marc A1 Chrastinová, Lenka A1 Vokurková, Jitka A1 Růžičková, Vladislava A1 Doškař, Jiří A1 Swings, Jean A1 Hájek, VáclavYR 2005 T1 Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys JF International Journal of Systematic and Evolutionary Microbiology, VO 55 IS 5 SP 1953 OP 1958 DO https://doi.org/10.1099/ijs.0.63590-0 PB Microbiology Society, SN 1466-5034, AB Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys (Saimiri sciureus L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and hsp60 gene sequencing, and from the most closely related species by using DNA–DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the Staphylococcus aureus species group (99 % similarity) but are biochemically similar to Staphylococcus piscifermentans, from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from d-mannitol, the inability to hydrolyse aesculin and DNA and the absence of α-glucosidase. On the basis of these analyses, a novel species of the genus Staphylococcus is described, for which the name Staphylococcus simiae sp. nov. is proposed, with CCM 7213T (=LMG 22723T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63590-0