1887

Abstract

Gammaproteobacteria belonging and related to the genus are an emerging group of complex carbohydrate-degrading marine bacteria. Previously, all of the representatives were placed within or were unclassified. Recently, a new genus, , represented by a single species, , was formed to include an endosymbiotic branch of these organisms. In this study, based on 16S rRNA gene sequence similarity and phenotypic analyses, a new genus, , is proposed to accommodate the most versatile marine carbohydrate degrader yet identified, gen. nov., sp. nov. 2-40 (=ATCC 43961=DSM 17024). strain 2-40 can degrade 10 tested complex polysaccharides: agar, alginate, chitin, cellulose, fucoidan, laminarin, pectin, pullulan, starch and xylan. 2-40 shares 90·5 % 16S rRNA gene sequence similarity with the type strain of the type species, IRE-31, and 91·5 % with T7902, and can be further distinguished from members of these two genera by 16S rRNA gene cluster analysis, the ability to utilize 10 different complex polysaccharides as sole carbon sources, a significantly lower G+C content and differences in fatty acid content. The three genera of complex polysaccharide-degrading, marine bacteria now encompass 20 strains from diverse marine niches.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63627-0
2005-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/4/ijs551545.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63627-0&mimeType=html&fmt=ahah

References

  1. Andrykovitch G., Marx I. 1988; Isolation of a new polysaccharide-digesting bacterium from a salt marsh. Appl Environ Microbiol 54:1061–1062
    [Google Scholar]
  2. Chenna R., Sugawara H., Koike T., Lopez R., Gibson T. J., Higgins D. G., Thompson J. D. 2003; Multiple sequence alignment with the clustal series of programs. Nucleic Acids Res 31:3497–3500 [CrossRef]
    [Google Scholar]
  3. Distel D. L., Morrill W., MacLaren-Toussaint N., Franks D., Waterbury J. 2002; Teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic γ -proteobacterium isolated from the gills of wood-boring molluscs (Bivalvia: Teredinidae. Int J Syst Evol Microbiol 52:2261–2269 [CrossRef]
    [Google Scholar]
  4. Ensor L., Stosz S., Weiner R. 1999; Expression of multiple complex polysaccharide-degrading enzyme systems by marine bacterium strain 2-40. J Ind Microbiol Biotechnol 23:123–126 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1988; Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet 22:521–565 [CrossRef]
    [Google Scholar]
  6. Gonzalez J. M., Weiner R. M. 2000; Phylogenetic characterization of marine bacterium strain 2-40, a degrader of complex polysaccharides. Int J Syst Evol Microbiol 50:831–834 [CrossRef]
    [Google Scholar]
  7. Gonzalez J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. 1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376 [CrossRef]
    [Google Scholar]
  8. Haygood M. G., Davidson S. K. 1997; Small-subunit rRNA genes and in situ hybridization with oligonucleotides specific for the bacterial symbionts in the larvae of the bryozoan Bugula neritina and proposal of “ Candidatus Endobugula sertula”. Appl Environ Microbiol 63:4612–4616
    [Google Scholar]
  9. Howard M. B., Ekborg N. A., Taylor L. E., Weiner R. M., Hutcheson S. W. 2003; Genomic analysis and initial characterization of the chitinolytic system of Microbulbifer degradans strain 2-40. J Bacteriol 185:3352–3360 [CrossRef]
    [Google Scholar]
  10. Imamura N., Nishijima M., Takadera T., Adachi K., Sakai M., Sano H. 1997; New anticancer antibiotics pelagiomicins, produced by a new marine bacterium Pelagiobacter variabilis . J Antibiot 50:8–12 [CrossRef]
    [Google Scholar]
  11. Kelly S., Coyne V., Sledjeski D., Fuqua W., Weiner R. 1990; Identification of a tyrosinase from a periphytic marine bacterium. FEMS Microbiol Lett 67:275–280 [CrossRef]
    [Google Scholar]
  12. Li L., Kato C., Horikoshi K. 1999a; Bacterial diversity in deep-sea sediments from different depths. Biodivers Conserv 8:659–677 [CrossRef]
    [Google Scholar]
  13. Li L., Guezennec J., Nichols P., Henry P., Yanagibayashi M., Kato C. 1999b; Microbial diversity in Nankai Trough sediments at a depth of 3,843 m. J Oceanogr 55:635–642 [CrossRef]
    [Google Scholar]
  14. Lim G. E., Haygood M. G. 2004; Candidatus Endobugula glebosa,” a specific bacterial symbiont of the marine bryozoan Bugula simplex . Appl Environ Microbiol 70:4921–4929 [CrossRef]
    [Google Scholar]
  15. Solano F., Sanchez-Amat A. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49:1241–1246 [CrossRef]
    [Google Scholar]
  16. Tanaka T., Yan L., Burgess J. G. 2003; Microbulbifer arenaceous sp. nov., a new endolithic bacterium isolated from the inside of red sandstone. Curr Microbiol 47:412–416
    [Google Scholar]
  17. Yoon J. H., Kim I. G., Shin D. Y., Kang K. H., Park Y. H. 2003a; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
    [Google Scholar]
  18. Yoon J. H., Kim H., Kang K. H., Oh T. K., Park Y. H. 2003b; Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov. Int J Syst Evol Microbiol 53:1357–1361 [CrossRef]
    [Google Scholar]
  19. Yoon J. H., Kim I. G., Oh T. K., Park Y. H. 2004; Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54:1111–1116 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63627-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63627-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error