1887

Abstract

A Gram-negative, non-spore-forming, short rod-shaped bacterium (UST010723-006) was isolated from the surface of the sponge in Hong Kong waters. Cells of UST010723-006 did not have flagella and were non-motile. Colonies were pale orange in colour, 2–4 mm in diameter, convex with a smooth surface and an entire translucent margin. Gas bubbles were observed in the colonies and also in the agar matrix underneath and adjacent to the colonies. UST010723-006 was heterotrophic, strictly aerobic and required NaCl for growth (2·0–6·0 %). It grew at pH 5·0–10·0 and between 12 and 44 °C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST010723-006 within the genus of the -subclass of the . The DNA G+C content is 40·6 mol% and the dominant fatty acids were 12 : 0 3-OH, 14 : 0, 15 : 0 iso 2-OH, 16 : 0, 16 : 17, 17 : 18 and 18 : 17 (altogether representing 75·9 % of the total).These data supported the affiliation of UST010723-006 to the genus . The closest relatives were , , and with similarity values ranging from 95·4 to 96·8 %. UST010723-006 differed from these closest relatives by 9–19 traits. Molecular evidence, together with phenotypic characteristics, suggests that UST010723-006 constitutes a novel species within the genus . The name sp. nov. is proposed for this bacterium. The type strain is UST010723-006 (=NRRL B-41100=JCM 12884).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63638-0
2005-07-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/4/ijs551593.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63638-0&mimeType=html&fmt=ahah

References

  1. Acar J. F. 1980; The disc susceptibility test. In Antibiotics in Laboratory and Medicine pp  24–54 Edited by Lorian V. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Egan S., Holmström C., Kjelleberg S. 2001; Pseudoalteromonas ulvae sp. nov., a bacterium with antifouling activities isolated from surface of a marine alga. Int J Syst Evol Microbiol 51:1499–1504
    [Google Scholar]
  3. Gauthier M. J. 1976; Alteromonas rubra sp. nov., a new marine antibiotic-producing bacterium. Int J Syst Bacteriol 26:459–466 [CrossRef]
    [Google Scholar]
  4. Gauthier M. J. 1982; Validation of the name Alteromonas luteoviolacea . Int J Syst Bacteriol 32:82–86 [CrossRef]
    [Google Scholar]
  5. Gauthier G., Gauthier M., Christen R. 1995; Phylogenetic analysis of the genera Alteromonas , Shewanella , and Moritella using genes for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45:755–761 [CrossRef]
    [Google Scholar]
  6. Grossart H. P., Steward G. F., Martinez J., Azam F. 2000; A simple, rapid method for demonstrating bacterial flagella. Appl Environ Microbiol 66:3632–3636 [CrossRef]
    [Google Scholar]
  7. Holmström C., James S., Neilan B. A., White D. C., Kjelleberg S. 1998; Pseudoalteromonas tunicata sp. nov., a bacterium that produces antifouling agents. Int J Syst Bacteriol 48:1205–1212 [CrossRef]
    [Google Scholar]
  8. Holmström C., Egan S., Franks S., McCloy S., Kjelleberg S. 2002; Antifouling activities expressed by marine surface associates Pseudoalteromonas species. FEMS Microbiol Ecol 41:47–58 [CrossRef]
    [Google Scholar]
  9. Isnansetyo A., Kamei Y. 2003; Pseudoalteromonas phenolica sp. nov., a novel marine bacterium that produces phenolic anti-methicillin-resistant Staphylococcus aureus substances. Int J Syst Evol Microbiol 53:583–588 [CrossRef]
    [Google Scholar]
  10. Ivanova E. P., Sawabe T., Lysenko A. M. 8 other authors 2002a; Pseudoalteromonas ruthenica sp. nov., isolated from marine invertebrates. Int J Syst Evol Microbiol 52:235–240
    [Google Scholar]
  11. Ivanova E. P., Shevchenko L. S., Sawabe T., Lysenko A. M., Svetashev V. I., Gorshkova N. M., Satomi M., Christen R., Mikhailov V. V. 2002b; Pseudoalteromonas maricaloris sp. nov., isolated from an Australian sponge, and reclassification of [ Pseudoalteromonas aurantia ] NCIMB 2033 as Pseudoalteromonas flavipulchra sp. nov. Int J Syst Evol Microbiol 52:263–271
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  13. MacDonell M. T., Singleton F. L., Hood M. A. 1982; Diluent composition for use of API 20E in characterizing marine and estuarine bacteria. Appl Environ Microbiol 44:423–427
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  15. Neu B., Voigt A., Mitlohner R. 7 other authors 2001; Biological cells as templates for hollow microcapsules. J Microencapsul 18:385–395 [CrossRef]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characteristics. In Methods for General and Molecular Biology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Techkarnjanaruk S., Pongpattanakitshote S., Goodman A. E. 1997; Use of a promoterless lacZ gene insertion to investigate chitinase gene expression in the marine bacterium Pseudoalteromonas sp. strain S9. Appl Environ Microbiol 63:2989–2996
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63638-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63638-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error