@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63660-0, author = "Wieser, Monika and Worliczek, Hanna and Kämpfer, Peter and Busse, Hans-Jürgen", title = "Bacillus herbersteinensis sp. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2005", volume = "55", number = "5", pages = "2119-2123", doi = "https://doi.org/10.1099/ijs.0.63660-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63660-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "pNA, p-nitroanilide", keywords = "pNP, p-nitrophenyl", abstract = "Two bacterial strains, designated D-1,5aT and D-1,5b, were isolated from a medieval wall painting in the chapel of Castle Herberstein, Styria (Austria). The Gram-positive, heterotrophic, aerobic, spore-forming rods showed nearly identical whole-cell protein patterns, identical genomic fingerprints and identical physiological profiles, demonstrating their relationship at the species level. Both strains contained meso-diaminopimelic acid in their peptidoglycan, possessed a quinone system comprising menaquinone MK-7 and had fatty acid profiles in which C15 : 0 iso and C15 : 0 anteiso were predominant. The 16S rRNA gene sequence of D-1,5aT showed the highest similarity (99·5 %) to the sequence of Bacillus sp. LMG 20243, and Bacillus flexus IFO 15715T was the next most closely related established species (96·5 %). Other type strains, such as Bacillus fastidiosus DSM 91T, Bacillus indicus SD/3T, Bacillus cibi JG-30T, Bacillus megaterium IAM 13418T, Bacillus cohnii DSM 6308T, Bacillus bataviensis LMG 21833T and Bacillus soli LMG 21838T, shared 96·0–96·1 % 16S rRNA gene sequence similarity with D-1,5aT. The combination of physiological and chemotaxonomic traits distinguishes the two strains from those species sharing the highest sequence similarities (96·0–96·5 %). On the basis of these characteristics and the phylogenetic position of strain D-1,5aT (=DSM 16534T=CCM 7228T), this strain is assigned as the type strain of a novel species of the genus Bacillus, for which the name Bacillus herbersteinensis sp. nov. is proposed.", }