RT Journal Article SR Electronic(1) A1 Wieser, Monika A1 Worliczek, Hanna A1 Kämpfer, Peter A1 Busse, Hans-JürgenYR 2005 T1 Bacillus herbersteinensis sp. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 55 IS 5 SP 2119 OP 2123 DO https://doi.org/10.1099/ijs.0.63660-0 PB Microbiology Society, SN 1466-5034, AB Two bacterial strains, designated D-1,5aT and D-1,5b, were isolated from a medieval wall painting in the chapel of Castle Herberstein, Styria (Austria). The Gram-positive, heterotrophic, aerobic, spore-forming rods showed nearly identical whole-cell protein patterns, identical genomic fingerprints and identical physiological profiles, demonstrating their relationship at the species level. Both strains contained meso-diaminopimelic acid in their peptidoglycan, possessed a quinone system comprising menaquinone MK-7 and had fatty acid profiles in which C15 : 0 iso and C15 : 0 anteiso were predominant. The 16S rRNA gene sequence of D-1,5aT showed the highest similarity (99·5 %) to the sequence of Bacillus sp. LMG 20243, and Bacillus flexus IFO 15715T was the next most closely related established species (96·5 %). Other type strains, such as Bacillus fastidiosus DSM 91T, Bacillus indicus SD/3T, Bacillus cibi JG-30T, Bacillus megaterium IAM 13418T, Bacillus cohnii DSM 6308T, Bacillus bataviensis LMG 21833T and Bacillus soli LMG 21838T, shared 96·0–96·1 % 16S rRNA gene sequence similarity with D-1,5aT. The combination of physiological and chemotaxonomic traits distinguishes the two strains from those species sharing the highest sequence similarities (96·0–96·5 %). On the basis of these characteristics and the phylogenetic position of strain D-1,5aT (=DSM 16534T=CCM 7228T), this strain is assigned as the type strain of a novel species of the genus Bacillus, for which the name Bacillus herbersteinensis sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63660-0