@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63720-0, author = "Singla, A. K. and Mayilraj, S. and Kudo, Takuji and Krishnamurthi, S. and Prasad, G. S. and Vohra, R. M.", title = "Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2005", volume = "55", number = "6", pages = "2561-2564", doi = "https://doi.org/10.1099/ijs.0.63720-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63720-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "An actinobacterial strain, RMV-1378T, isolated from a cold desert of the Indian Himalayas, was subjected to polyphasic taxonomic characterization. The strain formed branching, non-fragmenting vegetative hyphae and did not produce diffusible pigments. Neither aerial mycelium nor spore formation was observed. The G+C content of the DNA was 72·0 mol%. The strain had chemotaxonomic characteristics typical of the genus Actinoalloteichus and was closely related (99·3 % 16S rRNA gene sequence similarity) to Actinoalloteichus cyanogriseus, currently the only Actinoalloteichus species with a validly published name. However, the results of DNA–DNA hybridization experiments showed 51·9 % relatedness with the type strain of A. cyanogriseus. On the basis of the above data and the physiological and biochemical distinctiveness of RMV-1378T (=MTCC 6194T=JCM 12472T=DSM 44848T), this strain should be classified as the type strain of a novel species of Actinoalloteichus, for which the name Actinoalloteichus spitiensis sp. nov. is proposed.", }