RT Journal Article SR Electronic(1) A1 Singla, A. K. A1 Mayilraj, S. A1 Kudo, Takuji A1 Krishnamurthi, S. A1 Prasad, G. S. A1 Vohra, R. M.YR 2005 T1 Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas JF International Journal of Systematic and Evolutionary Microbiology, VO 55 IS 6 SP 2561 OP 2564 DO https://doi.org/10.1099/ijs.0.63720-0 PB Microbiology Society, SN 1466-5034, AB An actinobacterial strain, RMV-1378T, isolated from a cold desert of the Indian Himalayas, was subjected to polyphasic taxonomic characterization. The strain formed branching, non-fragmenting vegetative hyphae and did not produce diffusible pigments. Neither aerial mycelium nor spore formation was observed. The G+C content of the DNA was 72·0 mol%. The strain had chemotaxonomic characteristics typical of the genus Actinoalloteichus and was closely related (99·3 % 16S rRNA gene sequence similarity) to Actinoalloteichus cyanogriseus, currently the only Actinoalloteichus species with a validly published name. However, the results of DNA–DNA hybridization experiments showed 51·9 % relatedness with the type strain of A. cyanogriseus. On the basis of the above data and the physiological and biochemical distinctiveness of RMV-1378T (=MTCC 6194T=JCM 12472T=DSM 44848T), this strain should be classified as the type strain of a novel species of Actinoalloteichus, for which the name Actinoalloteichus spitiensis sp. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63720-0