1887

Abstract

Two Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strains, DSW-1 and DSW-21, were isolated from sea water and subjected to a polyphasic taxonomic study. They grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Strains DSW-1 and DSW-21 were characterized chemotaxonomically as containing MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and iso-C as the major fatty acids. Their DNA G+C content was 38 mol%. Strains DSW-1 and DSW-21 exhibited four nucleotide differences in their 16S rRNA gene sequences and possessed a mean DNA–DNA relatedness level of 78 %. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains DSW-1 and DSW-21 formed a distinct lineage within the family . The 16S rRNA gene sequences of strains DSW-1 and DSW-21 had similarity levels of less than 92·2 % to the sequences of other members of the family . There were some differences in phenotypic properties between the two strains and species, the nearest phylogenetic neighbours. On the basis of phenotypic, phylogenetic and genetic data, strains DSW-1 (=KCTC 12391=DSM 17200) and DSW-21 were classified in a novel genus and species, gen. nov., sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63817-0
2005-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/6/2323.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63817-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  3. Bowman J. P., McCammon S. A., Brown M. V., Nichols D. S., McMeekin T. A. 1997; Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63:3068–3078
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Glöckner F. O., Fuchs B. M., Amann R. 1999; Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65:3721–3726
    [Google Scholar]
  8. Johansen J. E., Nielsen P., Sjøholm C. 1999; Description of Cellulophaga baltica gen.nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [ Cytophaga ] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49:1231–1240 [CrossRef]
    [Google Scholar]
  9. Kirchman D. L. 2002; The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 39:91–100
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  11. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  14. Nedashkovskaya O. I., Suzuki M., Lysenko A. M., Snauwaert C., Vancanneyt M., Swings J., Vysotskii M., Mikhailov V. V. 2004; Cellulophaga pacifica sp. nov. Int J Syst Evol Microbiol 54:609–613 [CrossRef]
    [Google Scholar]
  15. Nedashkovskaya O. I., Kim S. B., Han S. K. 9 other authors 2005a; Winogradskyella thalassocola gen.nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov.,marine bacteria of the family Flavobacteriaceae . Int J Syst Evol Microbiol 55:49–55 [CrossRef]
    [Google Scholar]
  16. Nedashkovskaya O. I., Kim S. B., Lee K. H., Bae K. S., Frolova G. M., Mikhailov V. V., Kim I. S. 2005b; Pibocella ponti gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi . Int J Syst Evol Microbiol 55:177–181 [CrossRef]
    [Google Scholar]
  17. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S. 2005c; Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [ Cytophaga ] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov. Int J Syst Evol Microbiol 55:225–229 [CrossRef]
    [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Lee D. H., Lysenko A. M., Shevchenko L. S., Frolova G. M., Mikhailov V. V., Lee K. H., Bae K. S. 2005d; Roseivirga ehrenbergii gen. nov., sp. nov., a novel marine bacterium of the phylum ‘ Bacteroidetes ’, isolated from the green alga Ulva fenestrata . Int J Syst Evol Microbiol 55:231–234 [CrossRef]
    [Google Scholar]
  19. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S. 2005e; Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea . Int J Syst Evol Microbiol 55:375–378 [CrossRef]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Frolova G. M., Mikhailov V. V., Bae K. S., Lee D. H., Kim I. S. 2005f; Gramella echinicola gen. nov., sp. nov., a novel halophilic bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 55:391–394 [CrossRef]
    [Google Scholar]
  21. Reichenbach H. 1992; The order Cytophagales. In the Prokaryotes . . A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn. pp  3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  22. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI;
    [Google Scholar]
  23. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  24. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  26. Yi H., Yoon H. I., Chun J. 2005; Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int J Syst Evol Microbiol 55:409–416 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  29. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
  30. Yoon J.-H., Kang S.-J., Lee C.-H., Oh T.-K. 2005; Marinicola seohaensis gen. nov., sp. nov., isolated from sea water of the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:859–863 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63817-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63817-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error