1887

Abstract

A Gram-negative, non-motile, non-spore-forming, slightly halophilic bacterial strain, DSW-5, was isolated from seawater off Dokdo, Korea, and subjected to a polyphasic taxonomic study. It grew optimally at 25–28 °C and in the presence of 2 % (w/v) NaCl. Strain DSW-5 contained MK-6 as the predominant menaquinone and iso-C, iso-C and iso-C 3-OH as the major fatty acids. The major polar lipids detected were phosphatidylethanolamine, three unidentified phospholipids and an amino-group-containing lipid. The DNA G+C content was 30.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DSW-5 was most closely related to the genus . Similarity values between the 16S rRNA gene sequences of strain DSW-5 and the type strains of recognized species were in the range 96.2–96.8 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain DSW-5 (=KCTC 12392=DSM 17204) was classified in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

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2006-06-01
2024-04-16
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References

  1. Baumann P., Baumann L. 1981; The marine gram-negative eubacteria: genera Photobacterium Beneckea , Alteromonas , Pseudomonas and Alcaligenes . In The Prokaryotes , vol. 2 pp  1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. Berlin: Springer;
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen nov., sp. nov a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp.nov. and P. filamentus sp. nov.,gas vacuolated polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
    [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  7. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  8. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  9. McGuire A. J., Franzmann P. D., McMeekin T. A. 1987; Flectobacillus glomeratus sp. nov., a curved, nonmotile, pigmented bacterium isolated from antarctic marine environment. Syst Appl Microbiol 9:265–272 [CrossRef]
    [Google Scholar]
  10. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  11. Reichenbach H. 1992; The order Cytophagales. In The Prokaryotes . A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn. pp  3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  12. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note no: 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  13. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  14. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  15. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
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