1887

Abstract

The taxonomic status of two bacterial strains isolated from human blood was characterized using a polyphasic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genus and that produce C and C fatty acids on pyrolysis GC, and dihydrogenated menaquinone with nine isoprene units as the predominant isoprenologue. The generic assignment was confirmed by 16S rRNA gene sequencing. Comparative analysis of the 16S rRNA gene sequence showed that these isolates constitute a distinct phyletic line within the genus , displaying 96·2 and 97·2 % sequence similarities to and , respectively. The two isolates could be distinguished from the type strains of the latter species on the basis of several phenotypic traits. The genotypic and phenotypic data show that the strains merit classification as a novel species of , for which the name sp. nov. is proposed, with type strain IMMIB RIV-956 (=DSM 44902=CCUG 50873).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63856-0
2006-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/1/193.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63856-0&mimeType=html&fmt=ahah

References

  1. Becker, B., Lechevalier, M. P., Gordon, R. E. & Lechevalier, H. A.(1964). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12, 421–423. [Google Scholar]
  2. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F.(1978). Complete nucleotide sequence of a16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Gordon, R. E.(1966). Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45, 355–364.[CrossRef] [Google Scholar]
  7. Gordon, R. E.(1967). The taxonomy of soil bacteria. In The Ecology of Soil Bacteria, pp. 293–321. Edited by T. R. G. Gray & B. Parkinson. Liverpool: Liverpool University Press.
  8. Gordon, R. E. & Mihm, J. M.(1957). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27. [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Kämpfer, P., Andersson, M. A., Rainey, F. A., Kroppenstedt, R. M. & Salkinoja-Salonen, M.(1999).Williamsia muralis gen. nov., sp. nov., isolated from the indoor environment of a children's day care centre. Int J Syst Bacteriol 49, 681–687.[CrossRef] [Google Scholar]
  11. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  12. Lechevalier, M. P.(1968). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944. [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  14. Lechevalier, M. P., de Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  15. Ludwig, W., Strunk, O., Westram, R. & 29 other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  16. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M.(1980). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–223.[CrossRef] [Google Scholar]
  17. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Stach, J. E. M., Maldonado, L. A., Ward, A. C., Bull, A. T. & Goodfellow, M.(2004).Williamsia maris sp. nov., a novel actinomycete isolated from the Sea of Japan. Int J Syst Evol Microbiol 54, 191–194.[CrossRef] [Google Scholar]
  20. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  21. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef] [Google Scholar]
  22. Yassin, A. F.(1988).Chemotaxonomische Untersuchungen zur vereinfachten Differenzierung und Identifizierung von aeroben Aktinomyzeten und Mykobakterien. Inaugural-Dissertation zur Erlangung des Doktorgrades der Mathematische-Naturwissenschaftlichen Fakultät der Rheinischen Friedrich-Wilhelms-Universität Bonn (in German).
  23. Yassin, A. F., Binder, C. & Schaal, K. P.(1993a). Identification of mycobacterial isolates by thin-layer and capillary gas-liquid chromatography under diagnostic routine conditions. Zentralbl Bakteriol 278, 34–48.[CrossRef] [Google Scholar]
  24. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P.(1993b). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef] [Google Scholar]
  25. Yassin, A. F., Rainey, F. A., Brzezinka, H., Burghardt, J., Lee, H. J. & Schaal, K. P.(1995).Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45, 522–527.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63856-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63856-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error