1887

Abstract

A Gram-negative, microaerophilic helical rod, isolated from the gastric mucosa of a dog and designated strain JKM4, was subjected to a polyphasic taxonomic study. The tightly coiled organism, measuring 10–18 μm long and up to 1 μm wide, was motile by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril running along the external side of the helix. Strain JKM4 grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 30 and 37 °C, but not at 25 or 42 °C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus and showed > 97 % similarity to , and , three species previously isolated from the canine gastric mucosa. Protein profiling of strain JKM4 using SDS-PAGE revealed a pattern different from those of other species of mammalian gastric origin and from . Additionally, the urease gene sequence of strain JKM4 was different from those of urease genes of , , and ‘ Helicobacter heilmannii’. It is thus proposed that strain JKM4 (=LMG 23188) represents a novel species within this genus, sp. nov.

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2006-07-01
2024-03-29
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References

  1. Baele M., Devriese L. A., Haesebrouck F. 2001; Lactobacillus agilis is an important component of the pigeon crop flora. J Appl Microbiol 91:488–491 [CrossRef]
    [Google Scholar]
  2. Baele M., Van den Bulck K., Decostere A., Vandamme P., Hänninen M. L., Ducatelle R., Haesebrouck F. 2004; Multiplex PCR assay for differentiation of Helicobacter felis , H. bizzozeronii and H. salomonis . J Clin Microbiol 42:1115–1122 [CrossRef]
    [Google Scholar]
  3. Cattoli G., Van Vugt R., Zanoni R. G., Sanguinetti V., Chiocchetti R., Gualtieri M., Vandenbroucke-Grauls C. M., Gaastra W., Kusters J. G. 1999; Occurrence and characterization of gastric Helicobacter spp. in naturally infected dogs. Vet Microbiol 70:239–250 [CrossRef]
    [Google Scholar]
  4. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P. 1999; Classification of Alcaligenes faecalis -like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49:405–413 [CrossRef]
    [Google Scholar]
  5. Costas M., Pot B., Vandamme P., Kersters K., Owen R. J., Hill L. R. 1990; Interlaboratory comparative study of the numerical analysis of one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoretic protein patterns of Campylobacter strains. Electrophoresis 11:467–474 [CrossRef]
    [Google Scholar]
  6. Dewhirst F. E., Fox J. G., On S. L. 2000; Recommended minimal standards for describing new species of the genus Helicobacter . Int J Syst Evol Microbiol 50:2231–2237 [CrossRef]
    [Google Scholar]
  7. Eaton K. A., Dewhirst F. E., Radin M. J., Fox J. G., Paster B. J., Krakowka S., Morgan D. R. 1993; Helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. Int J Syst Bacteriol 43:99–106 [CrossRef]
    [Google Scholar]
  8. Fox J. G., Taylor N. S., Edmonds P., Brenner D. J. 1988; Campylobacter pylori subsp. mustelae subsp. nov., isolated from the gastric mucosa of ferrets ( Mustela putorius furo ), and an emended description of Campylobacter pylori . Int J Syst Bacteriol 38:367–370 [CrossRef]
    [Google Scholar]
  9. Germani Y., Dauga C., Duval P., Huerre M., Levy M., Pialoux G., Sansonetti P., Grimont P. A. 1997; Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in a human gastric biopsy. Res Microbiol 148:315–326 [CrossRef]
    [Google Scholar]
  10. Gruntar I., Mehle J., Krt B., Ocepek M., Gombac M., Cerne M., Pogacnik M. 2003; New approaches in isolation of Helicobacter spp. from gastric mucosa in dogs. Int J Med Microbiol 293 (Supplement), 65:
    [Google Scholar]
  11. Hänninen M. L., Happonen I., Saari S., Jalava K. 1996; Culture and characteristics of Helicobacter bizzozeronii , a new canine gastric Helicobacter sp. Int J Syst Bacteriol 46:160–166 [CrossRef]
    [Google Scholar]
  12. Hänninen M. L., Utriainen M., Happonen I., Dewhirst F. E. 2003; Helicobacter sp. flexispira 16S rDNA taxa 1, 4 and 5 and Finnish porcine Helicobacter isolates are members of the species Helicobacter trogontum (taxon 6. Int J Syst Evol Microbiol 53:425–433 [CrossRef]
    [Google Scholar]
  13. Houf K., On S. L. W., Coenye T., Mast J., Van Hoof J., Vandamme P. 2005; Arcobacter cibarius sp. nov., isolated from broiler carcasses. Int J Syst Evol Microbiol 55:713–717 [CrossRef]
    [Google Scholar]
  14. Jalava K., Kaartinen M., Utriainen M., Happonen I., Hänninen M. L. 1997; Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii . Int J Syst Bacteriol 47:975–982 [CrossRef]
    [Google Scholar]
  15. Jalava K., On S. L., Vandamme P. A., Happonen I., Sukura A., Hänninen M. L. 1998; Isolation and identification of Helicobacter spp. from canine and feline gastric mucosa. Appl Environ Microbiol 64:3998–4006
    [Google Scholar]
  16. Jalava K., On S. L., Harrington C. S., Andersen L. P., Hänninen M. L., Vandamme P. A. 2001; A cultured strain of “ Helicobacter heilmannii ”, a human gastric pathogen, identified as H. bizzozeronii : evidence for zoonotic potential of Helicobacter . Emerg Infect Dis 7:1036–1038 [CrossRef]
    [Google Scholar]
  17. Lee A., Hazell S. L., O'Rourke J., Kouprach S. 1988; Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun 56:2843–2850
    [Google Scholar]
  18. Lee A., Phillips M. W., O'Rourke J. L. 8 other authors; 1992; Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int J Syst Bacteriol 42:27–36 [CrossRef]
    [Google Scholar]
  19. On S. L. W., Bloch B., Holmes B., Hoste B., Vandamme P. 1995; Campylobacter hyointestinalis subsp. lawsonii subsp. nov., isolated from the porcine stomach, and an emended description of Campylobacter hyointestinalis . Int J Syst Bacteriol 45:767–774 [CrossRef]
    [Google Scholar]
  20. O'Rourke J. L., Solnick J. V., Neilan B. A., Seidel K., Hayter R., Hansen L. M., Lee A. 2004; Description of ‘ Candidatus Helicobacter heilmannii’ based on DNA sequence analysis of 16S rRNA and urease genes. Int J Syst Evol Microbiol 54:2203–2211 [CrossRef]
    [Google Scholar]
  21. Patterson M. M., Schrenzel M. D., Feng Y., Xu S., Dewhirst F. E., Paster B. J., Thibodeau S. A., Versalovic J., Fox J. G. 2000; Helicobacter aurati sp. nov., a urease-positive Helicobacter species cultured from gastrointestinal tissues of Syrian hamsters. J Clin Microbiol 38:3722–3728
    [Google Scholar]
  22. Pot B., Devriese L. A., Hommez J., Miry C., Vandemeulebroecke K., Kersters K., Haesebrouck F. 1994; Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals. J Appl Bacteriol 77:362–369 [CrossRef]
    [Google Scholar]
  23. Stanley J., Linton D., Burnens A. P., Dewhirst F. E., Owen R. J., Porter A., On S. L., Costas M. 1993; Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype. J Gen Microbiol 139:2495–2504 [CrossRef]
    [Google Scholar]
  24. Utriainen M., Jalava K., Sukura A., Hanninen M. L. 1997; Morphological diversity of cultured canine gastric Helicobacter spp. Comp Immunol Microbiol Infect Dis 20:285–297 [CrossRef]
    [Google Scholar]
  25. Vandamme P., Falsen E., Pot B., Hoste B., Kersters K., De Ley J. 1989; Identification of EF group 22 campylobacters from gastroenteritis cases as Campylobacter concisus . J Clin Microbiol 27:1775–1781
    [Google Scholar]
  26. Vandamme P., Pot B., Falsen E., Kersters K., De Ley J. 1990; Intra- and interspecific relationships of veterinary campylobacters revealed by numerical analysis of electrophoretic protein profiles and DNA: DNA hybridizations. Syst Appl Microbiol 13:295–303 [CrossRef]
    [Google Scholar]
  27. Vandamme P., Pot B., Kersters K. 1991; Differentiation of campylobacters and Campylobacter -like organisms by numerical analysis of one-dimensional electrophoretic protein patterns. Syst Appl Microbiol 14:57–66 [CrossRef]
    [Google Scholar]
  28. Vandamme P., Dewettinck D., Kersters K. 1992a; Application of numerical analysis of electrophoretic protein profiles for the identification of thermophilic campylobacters. Syst Appl Microbiol 15:402–408 [CrossRef]
    [Google Scholar]
  29. Vandamme P., Vancanneyt M., Pot B. & 10 other authors (1992b). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356 [CrossRef]
    [Google Scholar]
  30. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial systematics. Microb Rev 60:407–438
    [Google Scholar]
  31. Vandamme P., van Doorn L.-J., Al Rashid S. T., Quint W. G. V., van der Plas J., Chan V. L., On S. L. W. 1997; Campylobacter hyoilei Alderton et al . 1995 and Campylobacter coli Véron and Chatelain 1973 are subjective synonyms. Int J Syst Bacteriol 47:1055–1060 [CrossRef]
    [Google Scholar]
  32. Vandamme P., Harrington C. S., Jalava K., On S. L. 2000; Misidentifying helicobacters: the Helicobacter cinaedi example. J Clin Microbiol 38:2261–2266
    [Google Scholar]
  33. Van den Bulck K., Decostere A., Baele M., Driessen A., Debongnie J. C., Burette A., Stolte M., Ducatelle R., Haesebrouck F. 2005a; Identification of non- Helicobacter pylori spiral organisms in gastric samples from humans, dogs and cats. J Clin Microbiol 43:2256–2260 [CrossRef]
    [Google Scholar]
  34. Van den Bulck K., Decostere A., Gruntar I., Baele M., Krt B., Ducatelle R., Haesebrouck F. 2005b; In vitro antimicrobial susceptibility testing of Helicobacter felis , H. bizzozeronii and H. salomonis . Antimicrob Agents Chemother 49:2997–3000 [CrossRef]
    [Google Scholar]
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