@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63860-0, author = "Van den Bulck, K. and Decostere, A. and Baele, M. and Vandamme, P. and Mast, J. and Ducatelle, R. and Haesebrouck, F.", title = "Helicobacter cynogastricus sp. nov., isolated from the canine gastric mucosa", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2006", volume = "56", number = "7", pages = "1559-1564", doi = "https://doi.org/10.1099/ijs.0.63860-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63860-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-negative, microaerophilic helical rod, isolated from the gastric mucosa of a dog and designated strain JKM4T, was subjected to a polyphasic taxonomic study. The tightly coiled organism, measuring 10–18 μm long and up to 1 μm wide, was motile by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril running along the external side of the helix. Strain JKM4T grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 30 and 37 °C, but not at 25 or 42 °C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus Helicobacter and showed > 97 % similarity to Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis, three species previously isolated from the canine gastric mucosa. Protein profiling of strain JKM4T using SDS-PAGE revealed a pattern different from those of other Helicobacter species of mammalian gastric origin and from Helicobacter canis. Additionally, the urease gene sequence of strain JKM4T was different from those of urease genes of H. felis, H. bizzozeronii, H. salomonis and ‘Candidatus Helicobacter heilmannii’. It is thus proposed that strain JKM4T (=LMG 23188T) represents a novel species within this genus, Helicobacter cynogastricus sp. nov.", }