1887

Abstract

Rhizobial bacteria almost exclusively nodulate members of the families Fabaceae, Mimosaceae and Caesalpiniaceae, but are found on a single non-legume taxon, (Ulmaceae). Based on their host-range, their nitrogen-fixing ability and strain competition experiments, bacterial strains isolated from were thought to constitute a separate lineage that would account for their exceptional host affinity. This hypothesis was investigated by focusing on four isolates that are representative of the morphological and cultural types of -nodulating bradyrhizobia. Their evolutionary relationships with other rhizobia were analysed using 16S rRNA gene sequences and their nodulation properties were explored using the gene as a proxy for host-range specificity. Phylogenetic analyses of the 16S rRNA and gene sequences revealed that bacterial isolates from species are embedded among other bradyrhizobia. They did not cluster together in topologies based on the 16S rRNA or gene sequences, but were scattered among other bradyrhizobia belonging to either the or the lineages. These data suggest that the ability of some bradyrhizobia to nodulate species of the genus does not represent a historical relationship that predates the relationship between rhizobia and legumes, but is probably a more recent host switch for some rhizobia.

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2006-05-01
2024-03-29
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References

  1. Akkermans A. D. L., van Dijk C. 1981; Non-leguminous root-nodule symbiosis with actinomycetes and Rhizobium . In Nitrogen Fixation, vol. 1, Ecology pp  57–103 Edited by Broughton W. J. Oxford: Oxford University Press;
    [Google Scholar]
  2. Akkermans A. D. L., Abdulkadir S., Trinick M. J. 1978; N2-fixing root nodules in Ulmaceae: Parasponia or (and) Trema spp.?. Plant Soil 49:711–715 [CrossRef]
    [Google Scholar]
  3. Becking J. H. 1983; The Parasponia parviflora - Rhizobium symbiosis: host specificity, growth and nitrogen fixation under various conditions. Plant Soil 75:309–342 [CrossRef]
    [Google Scholar]
  4. Chaintreuil C., Boivin C., Dreyfus B., Giraud E. 2001; Characterization of the common nodulation genes of the photosynthetic Bradyrhizobium sp. ORS285 reveals the presence of a new insertion sequence upstream of nodA . FEMS Microbiol Lett 194:83–86 [CrossRef]
    [Google Scholar]
  5. Dénarié J., Debellé F., Promé J.-C. 1996; Rhizobium lipo-chitooligosaccharide nodulation factors: signaling molecules mediating recognition and morphogenesis. Annu Rev Biochem 65:503–535 [CrossRef]
    [Google Scholar]
  6. Dobert R. C., Breil B. T., Triplett E. W. 1994; DNA sequence of the common nodulation genes of Bradyrhizobium elkanii and their phylogenetic relationship to those of other nodulating bacteria. Mol Plant Microbe Interact 7:564–572 [CrossRef]
    [Google Scholar]
  7. Esseling J. J., Emons A. M. C. 2004; Dissection of Nod factor signalling in legumes: cell biology, mutants and pharmacological approaches. J Microsc 214:104–113 [CrossRef]
    [Google Scholar]
  8. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [CrossRef]
    [Google Scholar]
  9. Hannibal L., Lorquin J., D'Ortoli N. A., Garcia N., Chaintreuil C., Masson-Boivin C., Dreyfus B., Giraud E. 2000; Isolation and characterization of canthaxanthin biosynthesis genes from the photosynthetic bacterium Bradyrhizobium sp. strain ORS278. J Bacteriol 182:3850–3853 [CrossRef]
    [Google Scholar]
  10. Haukka K., Lindström K., Young J. P. W. 1998; Three phylogenetic groups of nodA and nifH genes in Sinorhizobium and Mesorhizobium isolates from leguminous trees growing in Africa and Latin America. Appl Environ Microbiol 64:419–426
    [Google Scholar]
  11. Keyser H. H., Hu T. S., Bohlool B. B., Weber D. F. 1982; Fast-growing rhizobia isolated from root nodules of soybean. Science 215:1631–1632 [CrossRef]
    [Google Scholar]
  12. Lafay B., Burdon J. J. 1998; Molecular diversity of rhizobia occurring on native shrubby legumes in southeastern Australia. Appl Environ Microbiol 64:3989–3997
    [Google Scholar]
  13. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959 [CrossRef]
    [Google Scholar]
  14. Mergaert P., Van Montagu M., Holsters M. 1997; Molecular mechanisms of Nod factor diversity. Mol Microbiol 25:811–817 [CrossRef]
    [Google Scholar]
  15. Moulin L., Béna G., Boivin-Masson C., Stepkowski T. 2004; Phylogenetic analyses of symbiotic nodulation genes support vertical and lateral gene co-transfer within the Bradyrhizobium genus. Mol Phylogenet Evol 30:720–732 [CrossRef]
    [Google Scholar]
  16. Pueppke S. G., Broughton W. J. 1999; Rhizobium sp. strain NGR 234 and R. fredii USDA257 share exceptionally broad, nested host ranges. Mol Plant Microbe Interact 12:293–318 [CrossRef]
    [Google Scholar]
  17. Ritsema T., Wijfjes A. H. M., Lugtenberg B. J. J., Spaink H. P. 1996; Rhizobium nodulation protein NodA is a host-specific determinant of the transfer of fatty acids in Nod factor biosynthesis. Mol Gen Genet 251:44–51
    [Google Scholar]
  18. Roche P., Maillet F., Plazanet C., Debellé F., Ferro M., Truchet G., Promé J. C., Dénarié J. 1996; The common nodABC genes of Rhizobium meliloti are host-range determinants. Proc Natl Acad Sci U S A 93:15305–15310 [CrossRef]
    [Google Scholar]
  19. Scott K. F. 1986; Conserved nodulation genes from the non-legume symbiont Bradyrhizobium sp. ( Parasponia ). Nucleic Acids Res 14:2905–2919 [CrossRef]
    [Google Scholar]
  20. Soepadmo E. 1977; Ulmaceae. In Flora Malesiana ser. 1 vol. 8 pp  31–76 Edited by van Steemis C. G. G. J. Jakarta: Noordhoff;
    [Google Scholar]
  21. Sritharan V., Barker R. H. J. 1991; A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR. Mol Cell Probes 5:385–395 [CrossRef]
    [Google Scholar]
  22. Suominen L., Roos C., Lortet G., Paulin L., Lindström K. 2001; Identification and structure of the Rhizobium galegae common nodulation genes: evidence for horizontal gene transfer. Mol Biol Evol 18:907–916 [CrossRef]
    [Google Scholar]
  23. Trinick M. J. 1973; Symbiosis between Rhizobium and the non-legume Trema aspera . Nature 244:459–460 [CrossRef]
    [Google Scholar]
  24. Trinick M. J. 1976; Rhizobium symbiosis with a non-legume. In First International Symposium on Nitrogen Fixation pp  507–517 Edited by Newton W. E., Nyman C. J. Pullman, WA: Washington State University Press;
    [Google Scholar]
  25. Trinick M. J. 1980; Growth of Parasponia in agar tube culture and symbiotic effectiveness of isolates from Parasponia spp. New Phytol 85:37–45 [CrossRef]
    [Google Scholar]
  26. Trinick M. J. 1988; Bradyrhizobium of the non-legume, Parasponia . In Microbiology in Action pp  107–118 Edited by Murrell W. G., Kennedy I. R. Letchworth, UK: Research Studies Press;
    [Google Scholar]
  27. Trinick M. J., Galbraith J. 1980; The Rhizobium requirements of the non-legume Parasponia in relationship to the cross-inoculation group concept of legumes. New Phytol 86:17–26 [CrossRef]
    [Google Scholar]
  28. Trinick M. J., Hadobas P. A. 1988; Biology of the Parasponia - Bradyrhizobium symbiosis. Plant Soil 110:177–185 [CrossRef]
    [Google Scholar]
  29. Trinick M. J., Hadobas P. A. 1989; Competition by Bradyrhizobium strains for nodulation of the non-legume Parasponia andersonii . Appl Environ Microbiol 55:1242–1248
    [Google Scholar]
  30. Ueda T., Suga Y., Yahiro N., Matsuguchi T. 1995; Phylogeny of Sym plasmids of rhizobia by PCR-based sequencing of a nodC segment. J Bacteriol 177:468–472
    [Google Scholar]
  31. Wernegreen J. J., Riley M. A. 1999; Comparison of the evolutionary dynamics of symbiotic and housekeeping loci: a case for the genetic coherence of rhizobial lineages. Mol Biol Evol 16:98–113 [CrossRef]
    [Google Scholar]
  32. Woese C. R. 2000; Interpreting the universal phylogenetic tree. Proc Natl Acad Sci U S A 97:8392–8396 [CrossRef]
    [Google Scholar]
  33. Young J. P. W., Haukka K. E. 1996; Diversity and phylogeny of rhizobia. New Phytol 133:87–94 [CrossRef]
    [Google Scholar]
  34. Young J. P. W., Johnston A. W. B. 1989; The evolution of specificity in the legume-rhizobium symbiosis. Trends Ecol Evol 4:341–349 [CrossRef]
    [Google Scholar]
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