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Abstract

Strain B33 is a moderately halophilic, exopolysaccharide-producing, Gram-negative, non-motile rod isolated from a hypersaline soil bordering a saline saltern on the Mediterranean seaboard in Murcia (Spain). The bacterium is chemoheterotrophic and strictly aerobic. It contains a pink pigment but does not synthesize bacteriochlorophyll . It requires 0·66 M Na, 0·1 M Mg and 0·1 M K for optimum growth. It does not produce acid from carbohydrates. It cannot grow with carbohydrates, organic acids, sugars, alcohols or amino acids as sole sources of carbon and energy. Its major fatty-acids are 18 : 17 (68·9 %) and 19 : 0 cyclo 8 (12·8 %). The sole respiratory lipoquinone found in strain B33 is ubiquinone-10. The DNA G+C content is 64·2 mol%. 16S rRNA gene sequence comparisons show that the isolate is a member of the clade within the class ‘’. The similarity values with and are 88·2 and 88·0 % respectively and 92·2 % with . DNA–DNA hybridization values with these species are <30 %. In the light of the polyphasic evidence gathered in this study it is proposed that the isolate be classified as a novel genus and species with the name gen. nov., sp. nov. The proposed type strain is strain B33 (=CECT 7066=LMG 22959).

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2005-11-01
2024-03-29
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