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Volume 56, Issue 2

Cachat and Priest 2005 is a later synonym of (Orla-Jensen 1919) Bergey . 1925 (Approved Lists 1980) Free

Abstract

Strain R0052, isolated from a North American dairy starter culture, was initially identified as based on phenotypic analyses. However, upon sequencing the 16S rRNA gene, it became clear that the isolate was very highly related to , and , as similarities ranging from 99·3 to 99·8 % were observed. As an initial screening test to investigate the relatedness of strain R0052 and reference strains of , and , the partial sequences for the genes encoding the alpha subunit of ATP synthase (), RNA polymerase alpha subunit (), phenylalanyl-tRNA synthase alpha subunit (), the translational elongation factor Tu (), a surface-layer protein () and the Hsp60 chaperonins () were determined and they revealed high relatedness between all of the strains. The determination of the 16S–23S rRNA internally transcribed spacer (ITS) sequences revealed 98·3–100 % similarity between and strains. SDS-PAGE of whole-cell proteins did not distinguish between these species. Fluorescent amplified fragment length polymorphism (FAFLP) could distinguish between these taxa, but they still constituted a single cluster within the group. Finally, DNA–DNA hybridization experiments between strain R0052 and the type strains of and yielded reassociation values above 70 % and confirmed that these names are synonyms.

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Keyword(s): FAFLP, fluorescent amplified fragment length polymorphism and ITS, internally transcribed spacer
SGM
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2006-02-01
2024-04-19
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References

  1. Antikainen J., Anton L., Sillanpää J., Korhonen T. K. 2002; Domains in the S-layer protein CbsA of Lactobacillus crispatus involved in adherence to collagens, laminin and lipoteichoic acids and in self-assembly. Mol Microbiol 46:381–394 [CrossRef]
     [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. (editors) 1925 Bergey's Manual of Determinative Bacteriology , 2nd edn. Baltimore: Williams & Wilkins;
     [Google Scholar]
  3. Boot H. J., Kolen C. P., Pot B., Kersters K., Pouwels P. H. 1996; The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus . Microbiology 142:2375–2384 [CrossRef]
     [Google Scholar]
  4. Cachat E., Priest F. G. 2005; Lactobacillus suntoryeus sp. nov., isolated from malt whisky distilleries. Int J Syst Evol Microbiol 55:31–34 [CrossRef]
     [Google Scholar]
  5. Dellaglio F., Felis G. E., Castioni A., Torriani S., Germond J. E. 2005; Lactobacillus delbrueckii subsp. indicus subsp. nov., isolated from Indian dairy products. Int J Syst Evol Microbiol 55:401–404 [CrossRef]
     [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  7. Fujisawa T., Benno Y., Yaeshima T., Mitsuoka T. 1992; Taxonomic study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and synonymy of Lactobacillus acidophilus group A3 (Johnson et al . 1980) with the type strain of Lactobacillus amylovorus (Nakamura 1981). Int J Syst Bacteriol 42:487–491 [CrossRef]
     [Google Scholar]
  8. Giraffa G., Gatti M., Rossetti L., Senini L., Neviani E. 2000; Molecular diversity within Lactobacillus helveticus as revealed by genotypic characterization. Appl Environ Microbiol 66:1259–1265 [CrossRef]
     [Google Scholar]
  9. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. 1998; Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [CrossRef]
     [Google Scholar]
  10. Guan L. L., Hagen K. E., Tannock G. W., Korver D. R., Fasenko G. M., Allison G. E. 2003; Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis. Appl Environ Microbiol 69:6750–6757 [CrossRef]
     [Google Scholar]
  11. Hagen K. E., Guan L. L., Tannock G. W., Korver D. R., Allison G. E. 2005; Detection, characterization, and in vitro and in vivo expression of genes encoding S-proteins in Lactobacillus gallinarum strains isolated from chicken crops. Appl Environ Microbiol 71:6633–6643 [CrossRef]
     [Google Scholar]
  12. Hammes W. P., Vogel R. F. 1995; The genus Lactobacillus . In The Lactic Acid Bacteria , vol. 2, The Genera of Lactic Acid Bacteria . pp  19–54 Edited by Wood B. J. B., Holzapfel W. H. London: Blackie Academic & Professional;
  13. Holzapfel W. H., Haberer P., Geisen R., Bjorkroth J., Schillinger U. 2001; Taxonomy and important features of probiotic microorganisms in food and nutrition. Am J Clin Nutr 73:365S–373S
     [Google Scholar]
  14. Kandler O., Weiss N. 1986; Genus Lactobacillus Beijerinck 1901. In Bergey's Manual of Systematic Bacteriology vol 2 pp  1209–1234 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  15. Klein G., Pack A., Bonaparte C., Reuter G. 1998; Taxonomy and physiology of probiotic lactic acid bacteria. Int J Food Microbiol 41:103–125 [CrossRef]
     [Google Scholar]
  16. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A. (editors) 1992 International Code of Nomenclature of Bacteria (1990 Revision) . Bacteriological Code Washington, DC: American Society for Microbiology;
     [Google Scholar]
  17. Lortal S. 1993; Crystalline surface-layers of the genus Lactobacillus . In Advances in Bacterial Paracrystalline Surface Layers pp  57–65 Edited by Beveridge T. J., Koval S. F. New York: Plenum;
     [Google Scholar]
  18. Naser S., Thompson F. L., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Thompson C. C., Vancanneyt M., Swings J. 2005a; Phylogeny and identification of enterococci by atpA gene sequence analysis. J Clin Microbiol 43:2224–2230 [CrossRef]
     [Google Scholar]
  19. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. 2005b; Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151:2141–2150 [CrossRef]
     [Google Scholar]
  20. Pitcher D. G., Saunders N. A., Owen R. G. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
     [Google Scholar]
  21. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods . Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
     [Google Scholar]
  22. Requena T., Burton J., Matsuki T., Munro K., Simon M. A., Tanaka R., Watanabe K., Tannock G. W. 2002; Identification, detection, and enumeration of human Bifidobacterium species by PCR targeting the transaldolase gene. Appl Environ Microbiol 68:2420–2427 [CrossRef]
     [Google Scholar]
  23. Sambrook J., Fritsch E., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  24. Sára M., Sleytr U. B. 2000; S-Layer proteins. J Bacteriol 182:859–868 [CrossRef]
     [Google Scholar]
  25. Sillanpää J., Martínez B., Antikainen J. 9 other authors 2000; Characterization of the collagen-binding S-layer protein CbsA of Lactobacillus crispatus . J Bacteriol 182:6440–6450 [CrossRef]
     [Google Scholar]
  26. Sleytr U. B., Sara M. 1997; Bacterial and archaeal S-layer proteins: structure-function relationships and their biotechnological applications. Trends Biotechnol 15:20–26 [CrossRef]
     [Google Scholar]
  27. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
     [Google Scholar]
  28. Stiles M. E., Holzapfel W. H. 1997; Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36:1–29 [CrossRef]
     [Google Scholar]
  29. Tannock G. W., Tilsala-Timisjarvi A., Rodtong S., Ng J., Munro K., Alatossava T. 1999; Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons. Appl Environ Microbiol 65:4264–4267
     [Google Scholar]
  30. Vancanneyt M., Neysens P., De Wachter M. 8 other authors 2005; Lactobacillus acidifarinae sp. nov. and Lactobacillus zymae sp. nov., from wheat sourdoughs. Int J Syst Evol Microbiol 55:615–620 [CrossRef]
     [Google Scholar]
  31. Ventura M., Callegari M. L., Morelli L. 2000; S-layer gene as a molecular marker for identification of Lactobacillus helveticus . FEMS Microbiol Lett 189:275–279 [CrossRef]
     [Google Scholar]
  32. Ventura M., Canchaya C., Meylan V., Klaenhammer T. R., Zink R. 2003; Analysis, characterization, and loci of the tuf genes in Lactobacillus and Bifidobacterium species and their direct application for species identification. Appl Environ Microbiol 69:6908–6922 [CrossRef]
     [Google Scholar]
  33. Walter J., Hertel C., Tannock G. W., Lis C. M., Munro K., Hammes W. P. 2001; Detection of Lactobacillus , Pediococcus , Leuconostoc , and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67:2578–2585 [CrossRef]
     [Google Scholar]
  34. Zeigler D. R. 2003; Gene sequences useful for predicting relatedness of whole genomes in bacteria. Int J Syst Evol Microbiol 53:1893–1900 [CrossRef]
     [Google Scholar]
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Lactobacillus suntoryeus Cachat and Priest 2005 is a later synonym of Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al. 1925 (Approved Lists 1980)
Int J Syst Evol Microbiol 56, 355 (2006); https://doi.org/10.1099/ijs.0.64001-0
/content/journal/ijsem/10.1099/ijs.0.64001-0
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