1887

Abstract

A yellow-pigmented, non-motile, Gram-negative bacterium, designated Fg 69, was isolated from a sediment sample collected in Chazhma Bay (Sea of Japan). The novel organism grew at 10–35 °C, was neutrophilic and required 3–10 % NaCl for optimal growth. Strain Fg 69 was able to degrade starch and to hydrolyse gelatin and Tween 80 weakly but not casein or agar. Predominant cellular fatty acids comprised n-C and n-C branched-chain and straight-chain saturated and unsaturated fatty acids, including iso-C (5 %), anteiso-C (11 %), C (9 %), iso-C (5 %), iso-C (8 %), C (5 %) and C 7 (5 %) and iso- and anteiso-branched 2-OH and 3-OH C to C fatty acids (26 % in total). The G+C content of the DNA was 40·4 mol%. 16S rRNA gene sequence data indicated that strain Fg 69 belonged to the genus but was distinct from recognized species (94–95 % sequence similarity). Based on these results, a novel species, sp. nov., is proposed. The type strain is Fg 69 (=KMM 6000=CIP 107843).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64087-0
2006-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/3/583.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64087-0&mimeType=html&fmt=ahah

References

  1. Azegami K., Nishiyama K., Watanabe Y., Kadota I., Ohuchi A., Fukazawa C. 1987; Pseudomonas plantarii sp. nov., the causal agent of rice seeding blight. Int J Syst Bacteriol 37:144–152 [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  3. Christie W. W. 1982 Lipid Analysis. Isolation, Separation, Identification and Structural Analysis of Lipids Oxford: Pergamon Press;
    [Google Scholar]
  4. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43:77–83 [CrossRef]
    [Google Scholar]
  5. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1993 phylip – Phylogenetic Inference Package, version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  8. Gascuel O. 1997; bionj, an improved version of the NJ algorithm based on a simple method of sequence data. Mol Biol Evol 14:685–695 [CrossRef]
    [Google Scholar]
  9. Ivanova E. P., Kiprianova E. A., Mikhailov V. V., Levanova F. G., Garagulya A. G., Gorshkova N. M., Yumoto N., Yoshikawa S. 1996; Characterization and identification of marine Alteromonas nigrifaciens strains and emendation of the description. Int J Syst Bacteriol 46:223–228 [CrossRef]
    [Google Scholar]
  10. Ivanova E. P., Gorshkova N. M., Sawabe T. 8 other authors 2004; Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish ( Stellaster equestris ) and sea grass ( Zostera marina . Int J Syst Evol Microbiol 54:475–480 [CrossRef]
    [Google Scholar]
  11. Ivanova E. P., Gorshkova N. M., Mikhailov V. V. 8 other authors 2005; Distribution of saprophytic bacteria in the atomic submarine accident zone in Chazhma Bay, Sea of Japan. Russ J Mar Biol (English translation of Biol Morya ) 31:65–72
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  14. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov., and Flavobacterium xanthum sp. nov., nom.rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063 [CrossRef]
    [Google Scholar]
  15. McMeekin T. A., Patterson J. T., Murray J. G. 1971; An initial approach to the taxonomy of some gram-negative yellow pigmented rods. J Appl Bacteriol 34:699–716 [CrossRef]
    [Google Scholar]
  16. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Vitellibacter vladivostokensis gen. nov., sp. nov. a new member of the phylum Cytophaga–Flavobacterium–Bacteroides . Int J Syst Evol Microbiol 53:1281–1286 [CrossRef]
    [Google Scholar]
  17. Nedashkovskaya O. I., Suzuki M., Vancanneyt M., Cleenwerck I., Zhukova N. V., Vysotskii M. V., Mikhailov V. V., Swings J. 2004; Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus . Int J Syst Evol Microbiol 54:1107–1110 [CrossRef]
    [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Mikhailov V. V., Bae K. S., Kim I. S. 2005; Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 55:235–238 [CrossRef]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64087-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64087-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error