1887

Abstract

A Gram-negative, rod-shaped, -like bacterial strain, DS-16, was isolated from soil from Dokdo, Korea, and subjected to a polyphasic taxonomic study. Strain DS-16 grew optimally at pH 6.0–7.0 and 30 °C in the presence of 0.5 % (w/v) NaCl. It contained Q-8 as the predominant ubiquinone and iso-C, iso-C and iso-C 9 as the major fatty acids. The DNA G+C content was 65.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-16 joined the cluster comprising species. The levels of 16S rRNA gene sequence similarity between strain DS-16 and the type strains of species ranged from 95.5 to 97.5 %. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness of strain DS-16, demonstrated that this novel strain differs from species with validly published names. On the basis of phenotypic, phylogenetic and genetic data, strain DS-16 (=KCTC 12543=CIP 108839) should be classified in the genus as a member of a novel species, for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64091-0
2006-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/6/1363.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64091-0&mimeType=html&fmt=ahah

References

  1. Assih E. A., Ouattara A. S., Thierry S., Cayol J.-L., Labat M., Macarie H. 2002; Stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 52:559–568
    [Google Scholar]
  2. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Finkmann W., Altendorf K., Stackebrandt E., Lipski A. 2000; Characterization of N2O-producing Xanthomonas -like isolates from biofilters as Stenotrophomonas nitritireducens sp.nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50:273–282 [CrossRef]
    [Google Scholar]
  5. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  6. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  7. Palleroni N. J. 1984; Genus Pseudomonas Migula 1894. In Bergey's Manual of Systematic Bacteriology vol. 1 pp  141–199 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Palleroni N. J., Bradbury J. F. 1993; Stenotrophomonas , a new bacterial genus for Xanthomonas maltophila (Hugh 1980, Swings et al . 1983). Int J Syst Bacteriol 43:606–609 [CrossRef]
    [Google Scholar]
  9. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI;
    [Google Scholar]
  10. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  11. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  12. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  13. Wolf A., Fritze A., Hagemann M., Berg G. 2002; Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties. Int J Syst Evol Microbiol 52:1937–1944 [CrossRef]
    [Google Scholar]
  14. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  15. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64091-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64091-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error