1887

Abstract

A rod-shaped marine bacterium, designated strain CL-TF09, isolated from a tidal flat in Ganghwa, Korea, was characterized based on its physiological and biochemical features, fatty acid profile and phylogenetic position. 16S rRNA gene sequence analysis revealed a clear affiliation with the family . Strain CL-TF09 showed the closest phylogenetic relationship with the genera and ; sequence similarities between CL-TF09 and the type strains of and species ranged from 90·7 to 91·8 %. Cells of strain CL-TF09 were non-motile and grew on solid media as yellow colonies. The strain grew in the presence of 1–5 % sea salts, within a temperature range of 5–30 °C and at pH 7–8. The strain had iso-C 3-OH (17·4 %), iso-C (16·7 %), anteiso-C (15·1 %) and iso-C 3-OH (13·4 %) as predominant fatty acids. The DNA G+C content was 33·9 mol%. Based on the physiological, fatty acid composition and phylogenetic data presented, strain CL-TF09 is considered to represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CL-TF09 (=KCCM 42118=JCM 13034).

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2006-04-01
2024-03-28
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  4. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp.nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia , Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55, 1471–1486 [CrossRef]
  5. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  6. Choi D. H., Kim Y. G., Hwang C. Y., Yi H., Chun J., Cho B. C. 2006; Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56:635–640 [CrossRef]
    [Google Scholar]
  7. Cole J. R., Chai B., Marsh T. L. 8 other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  8. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366
    [Google Scholar]
  9. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  10. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  11. Frette L., Jørgensen N. O. G., Irming H., Kroer N. 2004; Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54:519–524 [CrossRef]
    [Google Scholar]
  12. Garrity G. M., Holt J. G. 2001; The road map to the Manual. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  119–168 Edited by Boone D. R., Castenholtz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  13. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp.nov. and P. filamentus sp. nov.,gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
    [Google Scholar]
  14. Hansen G. H., Sørheim R. 1991; Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241 [CrossRef]
    [Google Scholar]
  15. Jooste P. J. 1985; The taxonomy and significance of Flavobacterium–Cytophaga strains from dairy sources . PhD thesis University of the Orange Free State;
  16. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  17. Kirchman D. L. 2002; The ecology of Cytophaga–Flavobacteria in aquatic environments. FEMS Microbiol Ecol 39:91–100
    [Google Scholar]
  18. Kumar S., Tamura K., Nei M. 2004; mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  19. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  20. Lyman J., Fleming R. H. 1940; Composition of sea water. J Mar Res 3:134–146
    [Google Scholar]
  21. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  24. Posada D., Crandall K. A. 1998; modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818 [CrossRef]
    [Google Scholar]
  25. Reichenbach H. 1992; The order Cytophagales. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn. pp  3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  27. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  28. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  29. Swofford D. L. 1998 paup*. Phylogenetic Analysis Using Parsimony, version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  30. Yoon J.-H., Kang S.-J., Oh T.-K. 2005; Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:793–798 [CrossRef]
    [Google Scholar]
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