1887

Abstract

A Gram-positive, rod-shaped, non-endospore-forming bacterium, strain ASB1, able to degrade tannin, was isolated from faeces of the Japanese large wood mouse, . Comparative analysis of the 16S rRNA gene sequence revealed that the strain could be assigned as a member of the genus . The nearest phylogenetic neighbours were determined as DSM 20602 (98.9 % 16S rRNA gene sequence similarity) and ASF 361 (98.9 %). Subsequent polyphasic analysis, including automated ribotyping and DNA–DNA hybridization experiments, confirmed that the isolate represents a novel species, for which the name sp. nov. is proposed. The DNA G+C content of the novel strain is 38.5 mol%. The cell-wall peptidoglycan is of type A4 -lys–-Asp. The type strain is ASB1 (=DSM 16634=CIP 108913).

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2006-07-01
2024-04-26
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References

  1. DSMZ 2001; Catalogue of Strains . , 7th edn.p– 617 Braunschweig: DSMZ; http://www.dsmz.de/species/murein.htm
  2. Felsenstein J. 1993 phylip – Phylogenetic inference package, version 3.5.1. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  3. Hammes W. P., Hertel C. 2003 The genera Lactobacillus and Carnobacterium . In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , release 3.15 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer; http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  4. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  5. Lane D. J. 1991; 16S-23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  125–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  6. Maidak B. L., Cole J. R., Lilburn T. G. 7 other authors 2001; The rdp-II (Ribosomal Database Project). Nucleic Acids Res 29:173–174 [CrossRef]
    [Google Scholar]
  7. Osawa R. 1990; Formation of a clear zone on tannin-treated brain heart infusion agar by Streptococcus sp. isolated from feces of koalas. Appl Environ Microbiol 56:829–831
    [Google Scholar]
  8. Osawa R., Fujisawa T., Sly L. I. 1995a; Streptococcus gallolyticus sp. nov.; gallate degrading organisms formerly assigned to Streptococcus bovis . Syst Appl Microbiol 18:74–78 [CrossRef]
    [Google Scholar]
  9. Osawa R., Rainey F., Fukisawa T., Lang E., Busse H. J., Walsh T. P., Stackebrandt E. 1995b; Lonepinella koalarum gen. nov., sp. nov., a new tannin-protein complex degrading bacterium. Syst Appl Microbiol 18368–373 [CrossRef]
    [Google Scholar]
  10. Pukall R., Brambilla E., Stackebrandt E. 1998; Automated fragment length analysis of fluorescently-labeled 16S rDNA after digestion with 4-base cutting restriction enzymes. J Microbiol Methods 32:55–63 [CrossRef]
    [Google Scholar]
  11. Pukall R., Buntefuss D., Fruhling A., Rohde M., Kroppenstedt R. M., Burghardt J., Lebaron P., Bernard L., Stackebrandt E. 1999; Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria . Int J Syst Bacteriol 49:513–519 [CrossRef]
    [Google Scholar]
  12. Sasaki E., Shimada T., Osawa R., Nishitani Y., Spring S., Lang E. 2005; Isolation of tannin-degrading bacteria isolated from feces of the Japanese large wood mouse, Apodemus speciosus , feeding on tannin-rich acorns. Syst Appl Microbiol 28:358–365 [CrossRef]
    [Google Scholar]
  13. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  14. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
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