1887

Abstract

Several -like organisms related to the complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximately the first 500 bp) rather than full 16S rRNA gene sequencing is often used to identify species. Partial 16S rRNA gene sequence analysis revealed 100 % similarity between 65 clinical isolates and sp. MCRO 6 (GenBank accession no. X93032). Even after sequencing the nearly full-length 16S rRNA gene, closest similarity was only 99.6 % to ATCC 19530. Sequencing of the nearly full-length 16S rRNA gene, the 16S–23S internal transcribed spacer region and the gene did not reveal genotypic identity with the type strains of , or . Although sequence analysis suggested that these clinical isolates represented a novel species, mycolic acid analysis by HPLC failed to distinguish them from . Therefore, phenotypic analysis including growth characterization, antibiotic susceptibility testing and biochemical testing was performed. These strains from clinical samples should be recognized as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AR30097 (=ATCC BAA-1242=DSM 44942).

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2006-06-01
2024-03-28
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