1887

Abstract

The taxonomic position of a lemon-yellow-pigmented actinobacterium, strain K22-21, isolated from a soil sample from Lahaul-Spiti Valley in the Indian Himalayas, was determined using a polyphasic approach. The strain had phenotypic and chemical properties that were consistent with its classification in the genus . Alignment of the 16S rRNA gene sequence of strain K22-21 with sequences from DSM 9580, DSM 14215 and DSM 12453 revealed similarities of 98.5, 96.8 and 96.6 %, respectively. However, the level of DNA–DNA relatedness between strain K22-21 and was 55.1 %. The novel strain could be distinguished from type strains of the three species of the genus using DNA–DNA relatedness and phenotypic data. Based on these differences, strain K22-21 (=MTCC 7154=DSM 17612) should be classified as the type strain of a novel species of , for which the name sp. nov. is proposed.

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2006-08-01
2024-03-28
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References

  1. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  2. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  3. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationery phases. J Liq Chromatogr 5:2359–2367 [CrossRef]
    [Google Scholar]
  4. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  5. Mayilraj S., Prasad G. S., Suresh K., Saini H. S., Shivaji S., Chakrabarti T. 2005; Planococcus stackebrandtii sp. nov., isolated from a cold desert of the Himalayas, India. Int J Syst Evol Microbiol 55:91–94 [CrossRef]
    [Google Scholar]
  6. Minnikin D. E., Goodfellow M. 1976; Lipid composition in the classification and identification of Nocardia and related taxa. In The Biology of the Nocardiaceae pp  160–219 Edited by Goodfellow M., Brownell G. H., Serrano J. A. London: Academic Press;
    [Google Scholar]
  7. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  8. Murray R. G. E., Raymond N., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp  21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Pandey K. K., Mayilraj S., Chakrabarti T. 2002; Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567 [CrossRef]
    [Google Scholar]
  10. Park Y. H., Suzuki K., Yim D. G. 7 other authors 1993; Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie van Leeuwenhoek 64:307–313
    [Google Scholar]
  11. Rainey F., Weiss N., Prauser H., Stackebrandt E. 1994; Further evidence for phylogenetic coherence of actinomycetes with group B-peptidoglycan and evidence for the phylogenetic intermixing of the genera Microbacterium and Aureobacterium as determined by 16S rDNA analysis. FEMS Microbiol Lett 118:135–139 [CrossRef]
    [Google Scholar]
  12. Saha P., Krishnamurthi S., Mayilraj S., Prasad G. S., Bora T. C., Chakrabarti T. 2005; Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. Int J Syst Evol Microbiol 55:1491–1495 [CrossRef]
    [Google Scholar]
  13. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Smith N. R., Gordon R. E., Clark F. E. 1952; Aerobic spore-forming bacteria . Agricultural Monograph no: 16 Washington, DC: US Department of Agriculture;
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Rainey F. A., Ward-Rainey N. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  18. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  19. Suzuki K., Sasaki J., Uramoto M., Nakase T., Komagata K. 1997; Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate ‘Curtobacterium psychrophilum ’ Inoue and Komagata 1976. Int J Syst Bacteriol 47:474–478 [CrossRef]
    [Google Scholar]
  20. Tourova T. P., Antonov A. S. 1987; Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19:333–355
    [Google Scholar]
  21. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Wieser M., Schumann P., Martin K., Altenburger P., Burghardt J., Lubitz W., Busse H.-J. 1999; Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria. Int J Syst Bacteriol 49:1165–1170 [CrossRef]
    [Google Scholar]
  24. Zlamala C., Schumann P., Kämpfer P., Rosselló-Mora R., Lubitz W., Busse H.-J. 2002; Agrococcus baldri sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52:1211–1216 [CrossRef]
    [Google Scholar]
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