1887

Abstract

A novel aerobic, Gram-negative bacterium, named 13IX/A01/164, was isolated from surface waters in the coastal north-western Mediterranean Sea. Cells were motile, straight rods, 2.5 μm long and 0.2 μm wide, and formed orange colonies on marine agar medium. The G+C content of the genomic DNA of strain 13IX/A01/164 was 42 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the phylum within the family . On the basis of 16S rRNA gene sequence comparison and physiological and biochemical characteristics, this isolate represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 13IX/A01/164 (=DSM 17893=CIP 109092=OOB 256).

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2006-08-01
2024-04-23
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References

  1. Agogué H., Casamayor E. O., Joux F. 8 other authors 2004; Comparison of samplers for the biological characterization of the sea surface microlayer. Limnol Oceanogr Methods 2:213–225 [CrossRef]
    [Google Scholar]
  2. Agogué H., Casamayor E. O., Bourrain M., Obernosterer I., Joux F., Herndl G. J., Lebaron P. 2005; A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems. FEMS Microbiol Ecol 54:269–280 [CrossRef]
    [Google Scholar]
  3. Alfredsson G. A., Kristjansson J. K., Hjörleifsdottir S., Stetter K. O. 1988; Rhodothermus marinus gen. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland. J Gen Microbiol 134:299–306
    [Google Scholar]
  4. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  5. Antón J., Oren A., Benlloch S., Rodríguez-Valera F., Amann R., Rosselló-Mora R. 2002; Salinibacter ruber gen. nov., sp. nov. a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52:485–491
    [Google Scholar]
  6. De Groote D., van Doorn L. J., Ducatelle R., Verschuuren A., Tilmant K., Quint W. G. V., Haesebrouck F., Vandamme P. 1999; Phylogenetic characterization of ‘ Candidatus Helicobacter bovis’, a new gastric helicobacter in cattle. Int J Syst Bacteriol 49:1707–1715 [CrossRef]
    [Google Scholar]
  7. Demirjian D. C., Moris-Varas F., Cassidy C. S. 2001; Enzymes from extremophiles. Curr Opin Chem Biol 5:144–151 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  10. Galtier N., Gouy M., Gautier C. 1996; seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12:543–548
    [Google Scholar]
  11. Huber R., Stetter K. O. 1992; The order Thermotogales . In The Prokaryotes , 2nd edn. vol IV pp  3809–3815 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  12. Hudson J. A., Schofield K. M., Morgan H. W., Daniel R. M. 1989; Thermonema lapsum gen. nov., sp. nov., a thermophilic gliding bacterium. Int J Syst Bacteriol 39:485–487 [CrossRef]
    [Google Scholar]
  13. Imhoff J. F., Süling J. 1996; The phylogenetic relationship among Ectothiorhodospiraceae : a reevaluation of their taxonomy on the basis of 16S rDNA analyses. Arch Microbiol 165:106–113 [CrossRef]
    [Google Scholar]
  14. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  16. McCammon S. A., Bowman J. P. 2000 Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov. and Flavobacterium xanthum sp. nov., nom.rev., and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063 [CrossRef]
  17. Powers E. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758
    [Google Scholar]
  18. Raguénès G., Christen R., Guézennec J., Pignet P., Barbier G. 1997; Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana . Int J Syst Bacteriol 47:989–995 [CrossRef]
    [Google Scholar]
  19. Rainey F. A., da Costa M. S. 2001; Order II. Thermales ord. nov. In Bergey's Manual of Systematic Bacteriology 2nd edn., vol. 1 ( The Archaea and the Deeply Branching and Phototrophic Bacteria)p– 403 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer-Verlag;
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Sako Y., Takai K., Ishida Y., Uchida A., Katayama Y. 1996; Rhodothermus obamensis sp. nov., a modern lineage of extremely thermophilic marine bacteria. Int J Syst Bacteriol 46:1099–1104 [CrossRef]
    [Google Scholar]
  22. Silva Z., Horta C., da Costa M. S., Chung A. P., Rainey F. A. 2000; Polyphasic evidence for the reclassification of Rhodothermus obamensis Sako et al . 1996 as a member of the species Rhodothermus marinus Alfredsson et al . 1988. Int J Syst Evol Microbiol 50:1457–1461 [CrossRef]
    [Google Scholar]
  23. Tenreiro S., Nobre M. F., Rainey F. A., Miguel C., da Costa M. S. 1997; Thermonema rossianum sp. nov., a new thermophilic and slightly halophilic species from saline hot spring in Naples, Italy. Int J Syst Bacteriol 47:122–126 [CrossRef]
    [Google Scholar]
  24. Wery N., Lesongeur F., Pignet P., Derennes V., Cambon-Bonavita M. A., Godfroy A., Barbier G. 2001a; Marinitoga camini gen. nov., sp. nov. a rod-shaped bacterium belonging to the order Thermotogales , isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51:495–504
    [Google Scholar]
  25. Wery N., Moricet J. M., Cueff V., Jean J., Pignet P., Lesongeur F., Cambon-Bonavita M. A., Barbier G. 2001b; Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51:1789–1796 [CrossRef]
    [Google Scholar]
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